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Applied and Environmental Microbiology, June 2003, p. 3469-3475, Vol. 69, No. 6
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.6.3469-3475.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Secondary Metabolites of Flustra foliacea and Their Influence on Bacteria

Lars Peters,1 Gabriele M. König,1* Anthony D. Wright,1 Rüdiger Pukall,2 Erko Stackebrandt,2 Leo Eberl,3 and Kathrin Riedel3

Institute for Pharmaceutical Biology, University of Bonn, D-53115 Bonn,1 German Collection of Microorganisms and Cell Cultures (DSMZ) GmbH, D-38124 Braunschweig,2 Department of Microbiology, Technical University of Munich, D-80290 Munich, Germany3

Received 2 December 2002/ Accepted 20 March 2003

The North Sea bryozoan Flustra foliacea was investigated to determine its secondary metabolite content. Gas chromatography-mass spectrometry analysis of a dichloromethane extract of the bryozoan enabled 11 compounds to be identified. Preparative high-performance liquid chromatography of the extract resulted in the isolation of 10 brominated alkaloids (compounds 1 to 10) and one diterpene (compound 11). All of these compounds were tested to determine their activities in agar diffusion assays against bacteria derived from marine and terrestrial environments. Compounds 1, 3 to 7, 10, and 11 exhibited significant activities against one or more marine bacterial strains originally isolated from F. foliacea but only weak activities against all of the terrestrial bacteria. By using the biosensors Pseudomonas putida(pKR-C12), P. putida(pAS-C8), and Escherichia coli(pSB403) the antagonistic effect on N-acyl-homoserine lactone-dependent quorum-sensing systems was investigated. Compounds 8 and 10 caused reductions in the signal intensities in these bioassays ranging from 50 to 20% at a concentration of 20 µg/ml.

* Corresponding author. Mailing address: Institute for Pharmaceutical Biology, University of Bonn, Nußallee 6, D-53115 Bonn, Germany. Phone: 49 228 733747. Fax: 49 228 733250. E-mail: g.koenig{at}uni-bonn.de.

Applied and Environmental Microbiology, June 2003, p. 3469-3475, Vol. 69, No. 6
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.6.3469-3475.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Krick, A., Kehraus, S., Eberl, L., Riedel, K., Anke, H., Kaesler, I., Graeber, I., Szewzyk, U., Konig, G. M. (2007). A Marine Mesorhizobium sp. Produces Structurally Novel Long-Chain N-Acyl-L-Homoserine Lactones. Appl. Environ. Microbiol. 73: 3587-3594 [Abstract] [Full Text]  
  • Uroz, S., Chhabra, S. R., Camara, M., Williams, P., Oger, P., Dessaux, Y. (2005). N-Acylhomoserine lactone quorum-sensing molecules are modified and degraded by Rhodococcus erythropolis W2 by both amidolytic and novel oxidoreductase activities. Microbiology 151: 3313-3322 [Abstract] [Full Text]  


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