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Applied and Environmental Microbiology, June 2003, p. 3569-3572, Vol. 69, No. 6
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.6.3569-3572.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Density-Dependent Sorting of Physiologically Different Cells of Vibrio parahaemolyticus

Tomohiko Nishino,* Binaya B. Nayak, and Kazuhiro Kogure

Ocean Research Institute, The University of Tokyo, Nakano, Tokyo 164-8639, Japan

Received 2 December 2002/ Accepted 12 March 2003

A pure bacterial culture is composed of clonal cells in different physiological states. Separation of those subpopulations is critical for further characterization and for understanding various processes in the cultured cells. We used density-dependent cell sorting with Percoll to separate subpopulations from cultures of a marine bacterium, Vibrio parahaemolyticus. Cells from cultures in the exponential and stationary phases were fractionated according to their buoyant density, and their culturability and ability to maintain culturability under low-temperature and low-nutrient stress (stress resistance) were determined. The buoyant density of the major portion of the cells decreased with culture age. The culturability of stationary-phase cells increased with increasing buoyant density, but that of exponential-phase cells did not. Stress resistance decreased with increasing buoyant density regardless of the growth phase. The results indicate that density-dependent cell sorting is useful for separating subpopulations of different culturabilities and stress resistances. We expect that this method will be a powerful tool for analyzing cells in various physiological states, such as the viable but nonculturable state.

* Corresponding author. Mailing address: Ocean Research Institute, The University of Tokyo, 1-15-1, Minamidai, Nakano, Tokyo 164-8639, Japan. Phone: 81 3 5351 6485. Fax: 81 3 5351 6482. E-mail: nishino{at}ori.u-tokyo.ac.jp.

Applied and Environmental Microbiology, June 2003, p. 3569-3572, Vol. 69, No. 6
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.6.3569-3572.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Inoue, K., Nishimura, M., Nayak, B. B., Kogure, K. (2007). Separation of Marine Bacteria according to Buoyant Density by Use of the Density-Dependent Cell Sorting Method. Appl. Environ. Microbiol. 73: 1049-1053 [Abstract] [Full Text]  


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