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Applied and Environmental Microbiology, June 2003, p. 3617-3625, Vol. 69, No. 6
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.6.3617-3625.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Mapping of Genomic Regions (Quantitative Trait Loci) Controlling Production and Quality in Industrial Cultures of the Edible Basidiomycete Pleurotus ostreatus

Luis M. Larraya, Mikel Alfonso, Antonio G. Pisabarro, and Lucía Ramírez*

Departamento de Producción Agraria, Universidad Pública de Navarra, E-31006 Pamplona, Spain

Received 16 December 2002/ Accepted 27 March 2003

Industrial production of the edible basidiomycete Pleurotus ostreatus (oyster mushroom) is based on a solid fermentation process in which a limited number of selected strains are used. Optimization of industrial mushroom production depends on improving the culture process and breeding new strains with higher yields and productivities. Traditionally, fungal breeding has been carried out by an empirical trial and error process. In this study, we used a different approach by mapping quantitative trait loci (QTLs) controlling culture production and quality within the framework of the genetic linkage map of P. ostreatus. Ten production traits and four quality traits were studied and mapped. The production QTLs identified explain nearly one-half of the production variation. More interestingly, a single QTL mapping to the highly polymorphic chromosome VII appears to be involved in control of all the productivity traits studied. Quality QTLs appear to be scattered across the genome and to have less effect on the variation of the corresponding traits. Moreover, some of the new hybrid strains constructed in the course of our experiments had production or quality values higher than those of the parents or other commercial strains. This approach opens the possibility of marker-assisted selection and breeding of new industrial strains of this fungus.

* Corresponding author. Mailing address: Departamento de Producción Agraria, Universidad Pública de Navarra, E-31006 Pamplona, Spain. Phone: (34) 948 169 130. Fax: (34) 948 169 732. E-mail: lramirez{at}unavarra.es.

Applied and Environmental Microbiology, June 2003, p. 3617-3625, Vol. 69, No. 6
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.6.3617-3625.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Pisabarro, A. G., Perez, G., Lavin, J. L., Ramirez, L. (2008). Genetic networks for the functional study of genomes. Brief Funct Genomic Proteomic 7: 249-263 [Abstract] [Full Text]  


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