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Applied and Environmental Microbiology, June 2003, p. 3640-3645, Vol. 69, No. 6
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.6.3640-3645.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Adhesion, Invasion, and Translocation Characteristics of Listeria monocytogenes Serotypes in Caco-2 Cell and Mouse Models

Ziad W. Jaradat and Arun K. Bhunia^*

Molecular Food Microbiology Laboratory, Department of Food Science, Purdue University, West Lafayette, Indiana 47907

Received 9 October 2002/ Accepted 5 March 2003

Adhesion is a crucial first step in Listeria monocytogenes pathogenesis. In this study, we examined how the adhesion properties of serotypes correlate with their invasion efficiencies in a cell culture model (Caco-2) and in a mouse model. Adhesion characteristics of all 13 serotypes of L. monocytogenes (25 strains) were analyzed, which yielded three distinct groups (P < 0.05) with high-, medium-, and low-level-adhesion profiles. The efficiency of these strains in invading the Caco-2 cell line was analyzed, which produced two groups; however, the overall correlation (R²) was only 0.1236. In the mouse bioassay, all selected strains, irrespective of their adhesion profiles, translocated to the liver and the spleen with almost equal frequencies that did not show any clear relationship with adhesion profiles. However, the serotypes with increased adhesion showed a slightly increased translocation to the brain (R² = 0.3371). Collectively, these results indicate that an in vitro adhesion profile might not be an accurate assessment of a strain's ability to invade a cultured cell line or organs or tissues in a mouse model.

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* Corresponding author. Mailing address: Department of Food Science, 745 Agriculture Mall Dr., Purdue University, West Lafayette, IN 47907-2009. Phone: (765) 494-5443. Fax: (765) 494-7953. E-mail: bhuniaa{at}foodsci.purdue.edu.

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Applied and Environmental Microbiology, June 2003, p. 3640-3645, Vol. 69, No. 6
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.6.3640-3645.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

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