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Applied and Environmental Microbiology, June 2003, p. 3653-3657, Vol. 69, No. 6
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.6.3653-3657.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

On the Origins of Cyanuric Acid Hydrolase: Purification, Substrates, and Prevalence of AtzD from Pseudomonas sp. Strain ADP

Isaac Fruchey,1,{dagger} Nir Shapir,1,2,3 Michael J. Sadowsky,1,3,4 and Lawrence P. Wackett1,2,4*

Department of Biochemistry, Molecular Biology and Biophysics,2 BioTechnology Institute,1 Center for Microbial and Plant Genomics,4 Department of Soil, Water and Climate, University of Minnesota, St. Paul, Minnesota 551083

Received 6 January 2003/ Accepted 6 March 2003

Cyanuric acid hydrolase (AtzD) from Pseudomonas sp. strain ADP was purified to homogeneity. Of 22 cyclic amides and triazine compounds tested, only cyanuric acid and N-methylisocyanuric acid were substrates. Other cyclic amidases were found not to hydrolyze cyanuric acid. Ten bacteria that use cyanuric acid as a sole nitrogen source for growth were found to contain either atzD or trzD, but not both genes.

* Corresponding author. Mailing address: Department of Biochemistry, Molecular Biology and Biophysics, 140 Gortner Lab, University of Minnesota, 1479 Gortner Ave., St. Paul, MN 55108. Phone: (612) 625-3785. Fax: (612) 625-5780. E-mail: wackett{at}biosci.cbs.umn.edu.

{dagger} Present address: Battelle Memorial Institute, Aberdeen, MD 21001.

Applied and Environmental Microbiology, June 2003, p. 3653-3657, Vol. 69, No. 6
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.6.3653-3657.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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