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Applied and Environmental Microbiology, June 2003, p. 3681-3685, Vol. 69, No. 6
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.6.3681-3685.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Generation of Food-Grade Lactococcal Starters Which Produce the Lantibiotics Lacticin 3147 and Lacticin 481

Lisa O'Sullivan,1,2 Maire P. Ryan,1,2 R. Paul Ross,1* and Colin Hill2,3

Dairy Products Research Centre, Teagasc, Moorepark, Fermoy,1 Department of Microbiology,2 National Food Biotechnology Centre, University College Cork, County Cork, Republic of Ireland3

Received 7 September 2002/ Accepted 25 February 2003

Transconjugant lactococcal starters which produce both lantibiotics lacticin 3147 and lacticin 481 were generated via conjugation of large bacteriocin-encoding plasmids. A representative of one of the resultant strains proved more effective at killing Lactobacillus fermentum and inhibiting the growth of Listeria monocytogenes LO28H than either of the single bacteriocin-producing parental strains, demonstrating the potential of these transconjugants as protection cultures for food safety applications.

* Corresponding author. Mailing address: Dairy Quality Department, Dairy Products Research Centre, Moorepark, Fermoy, Co. Cork, Ireland. Phone: 353-25-42229. Fax: 353-25-42340. E-mail: pross{at}moorepark.teagasc.ie.

Applied and Environmental Microbiology, June 2003, p. 3681-3685, Vol. 69, No. 6
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.6.3681-3685.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.





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