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Applied and Environmental Microbiology, July 2003, p. 4260-4262, Vol. 69, No. 7
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.7.4260-4262.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Visualization of an Endophytic Streptomyces Species in Wheat Seed

Justin T. Coombs and Christopher M. M. Franco*

Department of Medical Biotechnology, Flinders University, Bedford Park, South Australia 5042, Australia

Received 28 October 2002/ Accepted 26 March 2003

Endophytic filamentous actinobacteria were isolated from surface-sterilized roots of wheat plants. Endophytic colonization of germinating wheat seed was examined using one of these endophytes, Streptomyces sp. strain EN27, tagged with the egfp gene. Endophytic colonization was observed from a very early stage of plant development with colonization of the embryo, endosperm, and emerging radicle.

* Corresponding author. Mailing address: Department of Medical Biotechnology, Flinders University, Bedford Park, South Australia 5042, Australia. Phone: 61 8 8204 5764. Fax: 61 8 8204 4101. E-mail: Chris.Franco{at}flinders.edu.au.

Applied and Environmental Microbiology, July 2003, p. 4260-4262, Vol. 69, No. 7
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.7.4260-4262.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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