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Applied and Environmental Microbiology, July 2003, p. 4285-4290, Vol. 69, No. 7
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.7.4285-4290.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Improved Adaptation to Cold-Shock, Stationary-Phase, and Freezing Stresses in Lactobacillus plantarum Overproducing Cold-Shock Proteins

Sylviane Derzelle,{dagger} Bernard Hallet, Thierry Ferain,{ddagger} Jean Delcour, and Pascal Hols*

Institut des Sciences de la Vie/Unité de Génétique, Université catholique de Louvain, B-1348 Louvain-la-Neuve, Belgium

Received 9 December 2002/ Accepted 23 April 2003

We have investigated the effect of overproducing each of the three cold shock proteins (CspL, CspP, and CspC) in the mesophilic lactic acid bacterium Lactobacillus plantarum NC8. CspL overproduction transiently alleviated the reduction in growth rate triggered by exposing exponentially growing cells to cold shock (8°C), suggesting that CspL is involved in cold adaptation. The strain overproducing CspC resumed growth more rapidly when stationary-phase cultures were diluted into fresh medium, indicating a role in the adaptation and recovery of nutritionally deprived cells. Overproduction of CspP led to an enhanced capacity to survive freezing.

* Corresponding author. Mailing address: Unité de Génétique, Université catholique de Louvain, 5 Place Croix du Sud, B-1348 Louvain-la-Neuve, Belgium. Phone: 32-10-478896. Fax: 32-10-473109. E-mail: hols{at}gene.ucl.ac.be.

{dagger} Present address: Génétique des Génomes Bactériens, Institut Pasteur, F-75724 Paris cedex 15, France.

{ddagger} Present address: Euroscreen S.A., B-1070 Brussels, Belgium.

Applied and Environmental Microbiology, July 2003, p. 4285-4290, Vol. 69, No. 7
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.7.4285-4290.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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