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Applied and Environmental Microbiology, July 2003, p. 4320-4324, Vol. 69, No. 7
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.7.4320-4324.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Oligonucleotide Probes That Detect Quantitatively Significant Groups of Butyrate-Producing Bacteria in Human Feces

Georgina L. Hold,^1* Andreas Schwiertz,^2, Rustam I. Aminov,¹ Michael Blaut,² and Harry J. Flint¹

Rowett Research Institute, Bucksburn, Aberdeen AB21 9SB, United Kingdom,¹ Deutsches Institut für Ernaehrungsforschung, Abteilung Gastrointestinale Mikrobiologie, 14558 Bergholz-Rehbrücke, Germany²

Received 30 December 2002/ Accepted 25 February 2003

16S rRNA-targeted oligonucleotide probes were designed for butyrate-producing bacteria from human feces. Three new cluster-specific probes detected bacteria related to Roseburia intestinalis, Faecalibacterium prausnitzii, and Eubacterium hallii at mean populations of 2.3, 3.8, and 0.6%, respectively, in samples from 10 individuals. Additional species-level probes accounted for no more than 1%, with a mean of 7.7%, of the total human fecal microbiota identified as butyrate producers in this study. Bacteria related to E. hallii and the genera Roseburia and Faecalibacterium are therefore among the most abundant known butyrate-producing bacteria in human feces.

Present address: SymbioHerborn Group, D-35745 Herborn, Germany.

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