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Applied and Environmental Microbiology, August 2003, p. 4575-4582, Vol. 69, No. 8
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.8.4575-4582.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Engineering Deinococcus geothermalis for Bioremediation of High-Temperature Radioactive Waste Environments

Hassan Brim,1 Amudhan Venkateswaran,1 Heather M. Kostandarithes,2 James K. Fredrickson,2 and Michael J. Daly1*

Department of Pathology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814,1 Pacific Northwest National Laboratory, Richland, Washington 993522

Received 5 May 2003/ Accepted 4 June 2003

Deinococcus geothermalis is an extremely radiation-resistant thermophilic bacterium closely related to the mesophile Deinococcus radiodurans, which is being engineered for in situ bioremediation of radioactive wastes. We report that D. geothermalis is transformable with plasmids designed for D. radiodurans and have generated a Hg(II)-resistant D. geothermalis strain capable of reducing Hg(II) at elevated temperatures and in the presence of 50 Gy/h. Additionally, D. geothermalis is capable of reducing Fe(III)-nitrilotriacetic acid, U(VI), and Cr(VI). These characteristics support the prospective development of this thermophilic radiophile for bioremediation of radioactive mixed waste environments with temperatures as high as 55°C.

* Corresponding author. Mailing address: Uniformed Services University of the Health Sciences (USUHS), Rm. B3153, 4301 Jones Bridge Rd., Bethesda, MD 20814-4799. Phone: (301) 295-3750. Fax: (301) 295-1640. E-mail: mdaly{at}usuhs.mil.

Applied and Environmental Microbiology, August 2003, p. 4575-4582, Vol. 69, No. 8
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.8.4575-4582.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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