AEM Tips for Better Browsing
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Brim, H.
Right arrow Articles by Daly, M. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Brim, H.
Right arrow Articles by Daly, M. J.
Agricola
Right arrow Articles by Brim, H.
Right arrow Articles by Daly, M. J.
Applied and Environmental Microbiology, August 2003, p. 4575-4582, Vol. 69, No. 8
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.8.4575-4582.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Engineering Deinococcus geothermalis for Bioremediation of High-Temperature Radioactive Waste Environments

Hassan Brim,1 Amudhan Venkateswaran,1 Heather M. Kostandarithes,2 James K. Fredrickson,2 and Michael J. Daly1*

Department of Pathology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814,1 Pacific Northwest National Laboratory, Richland, Washington 993522

Received 5 May 2003/ Accepted 4 June 2003

Deinococcus geothermalis is an extremely radiation-resistant thermophilic bacterium closely related to the mesophile Deinococcus radiodurans, which is being engineered for in situ bioremediation of radioactive wastes. We report that D. geothermalis is transformable with plasmids designed for D. radiodurans and have generated a Hg(II)-resistant D. geothermalis strain capable of reducing Hg(II) at elevated temperatures and in the presence of 50 Gy/h. Additionally, D. geothermalis is capable of reducing Fe(III)-nitrilotriacetic acid, U(VI), and Cr(VI). These characteristics support the prospective development of this thermophilic radiophile for bioremediation of radioactive mixed waste environments with temperatures as high as 55°C.

* Corresponding author. Mailing address: Uniformed Services University of the Health Sciences (USUHS), Rm. B3153, 4301 Jones Bridge Rd., Bethesda, MD 20814-4799. Phone: (301) 295-3750. Fax: (301) 295-1640. E-mail: mdaly{at}usuhs.mil.

Applied and Environmental Microbiology, August 2003, p. 4575-4582, Vol. 69, No. 8
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.8.4575-4582.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. Microbiol. Mol. Biol. Rev. Eukaryot. Cell All ASM Journals

Copyright © 2003 by the American Society for Microbiology. All rights reserved.