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Applied and Environmental Microbiology, August 2003, p. 4583-4594, Vol. 69, No. 8
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.8.4583-4594.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Microbial Quality and Direct PCR Identification of Lactic Acid Bacteria and Nonpathogenic Staphylococci from Artisanal Low-Acid Sausages

T. Aymerich,* B. Martín, M. Garriga, and M. Hugas

Meat Technology Center, IRTA, Granja Camps i Armet, Monells, Girona, Spain

Received 17 December 2002/ Accepted 28 May 2003

Detection of six species of lactic acid bacteria and six species of gram-positive catalase-positive cocci from low-acid fermented sausages (fuets and chorizos) was assessed by species-specific PCR. Without enrichment, Lactobacillus sakei and Lactobacillus curvatus were detected in 11.8% of the samples, and Lactobacillus plantarum and Staphylococcus xylosus were detected in 17.6%. Enriched samples allowed the detection of L. sakei and S. xylosus in all of the samples (100%) and of Enterococcus faecium in 11.8% of the sausages. The percentages of L. curvatus, L. plantarum, Staphylococcus carnosus, and Staphylococcus epidermidis varied depending on the sausage type. L. curvatus was detected in 80% of fuets and in 57% of chorizos. L. plantarum was found in 50% of fuets and 100% of chorizos. S. epidermidis was detected in only 11.8% of fuets, and S. carnosus was detected in only 5.9% of chorizos. Lactococcus lactis, Staphylococcus warneri, and Staphylococcus simulans were not detected in any sausage type. From a microbiological point of view, 70.6% of the samples could be considered of high quality, as they had low counts of Enterobacteriaceae and did not contain any of the food-borne pathogens assayed.

* Corresponding author. Mailing address: Meat Technology Center, IRTA, Granja Camps i Armet, Monells, Girona, Spain. Phone: 34 972 63 00 52. Fax: 34 972 63 03 73. E-mail: teresa.aymerich{at}irta.es.

Applied and Environmental Microbiology, August 2003, p. 4583-4594, Vol. 69, No. 8
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.8.4583-4594.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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