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Applied and Environmental Microbiology, August 2003, p. 4985-4988, Vol. 69, No. 8
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.8.4985-4988.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Development of a Sensitive Gene Expression Reporter System and an Inducible Promoter-Repressor System for Clostridium acetobutylicum

Laurence Girbal,1* Isabelle Mortier-Barrière,2 Frédéric Raynaud,1 Céline Rouanet,1 Christian Croux,1 and Philippe Soucaille1

Centre de Bioingénierie Gilbert Durand, Laboratoire de Biotechnologie-Bioprocédés, UMR CNRS 5504, UMR INRA 792, INSA,1 CRT/CRITT-Bioindustries, INSA, DGBA, 31077 Toulouse cedex 4, France2

Received 23 December 2002/ Accepted 2 June 2003

A sensitive gene expression reporter system was developed for Clostridium acetobutylicum ATCC 824 by using a customized gusA expression cassette. In discontinuous cultures, time course profiles of ß-glucuronidase specific activity reflected adequately in vivo dynamic up- and down-regulation of acidogenesis- and/or solventogenesis-associated promoter expression in C. acetobutylicum. Furthermore, a new inducible gene expression system was developed in C. acetobutylicum, based on the Staphylococcus xylosus xylose operon promoter-repressor regulatory system.

* Corresponding author. Mailing address: Centre de Bioingénierie Gilbert Durand, Laboratoire de Biotechnologie-Bioprocédés, UMR CNRS 5504, UMR INRA 792, INSA, 135 Avenue de Rangueil, 31077 Toulouse cedex 4, France. Phone: 33 5 61 55 94 19. Fax: 33 5 61 55 94 00. E-mail: girbal{at}insa-tlse.fr.

Applied and Environmental Microbiology, August 2003, p. 4985-4988, Vol. 69, No. 8
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.8.4985-4988.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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Copyright © 2003 by the American Society for Microbiology. All rights reserved.