This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Goode, D.
Right arrow Articles by Barrow, P. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Goode, D.
Right arrow Articles by Barrow, P. A.
Agricola
Right arrow Articles by Goode, D.
Right arrow Articles by Barrow, P. A.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, August 2003, p. 5032-5036, Vol. 69, No. 8
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.8.5032-5036.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Reduction of Experimental Salmonella and Campylobacter Contamination of Chicken Skin by Application of Lytic Bacteriophages

D. Goode,1 V. M. Allen,2 and P. A. Barrow3*

Oxford Brookes University, Headington, Oxford OX3 0BP,1 Department of Clinical Veterinary Science, University of Bristol, Langford, North Somerset BS40 5DU,2 Institute for Animal Health, Compton Laboratory, Compton, Newbury, Berkshire RG20 7NN, United Kingdom3

Received 2 December 2002/ Accepted 2 June 2003

Lytic bacteriophages, applied to chicken skin that had been experimentally contaminated with Salmonella enterica serovar Enteritidis or Campylobacter jejuni at a multiplicity of infection (MOI) of 1, increased in titer and reduced the pathogen numbers by less than 1 log10 unit. Phages applied at a MOI of 100 to 1,000 rapidly reduced the recoverable bacterial numbers by up to 2 log10 units over 48 h. When the level of Salmonella contamination was low (< log10 2 per unit area of skin) and the MOI was 105, no organisms were recovered. By increasing the number of phage particles applied (i.e., MOI of 107), it was also possible to eliminate other Salmonella strains that showed high levels of resistance because of restriction but to which the phages were able to attach.

* Corresponding author. Mailing address: Institute for Animal Health, Compton Laboratory, Compton, Newbury, Berkshire RG20 7NN, United Kingdom. Phone: 44-1635-578411. Fax: 44-1635-577243. E-mail: Paul.Barrow{at}bbsrc.ac.uk.

Applied and Environmental Microbiology, August 2003, p. 5032-5036, Vol. 69, No. 8
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.8.5032-5036.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Sirsat, S. A., Muthaiyan, A., Ricke, S. C. (2009). Antimicrobials for foodborne pathogen reduction in organic and natural poultry production. J. Appl. Poult. Res. 18: 379-388 [Abstract] [Full Text]  
  • Guenther, S., Huwyler, D., Richard, S., Loessner, M. J. (2009). Virulent Bacteriophage for Efficient Biocontrol of Listeria monocytogenes in Ready-To-Eat Foods. Appl. Environ. Microbiol. 75: 93-100 [Abstract] [Full Text]  
  • Oliver, S. P., Patel, D. A., Callaway, T. R., Torrence, M. E. (2009). ASAS Centennial Paper: Developments and future outlook for preharvest food safety. J ANIM SCI 87: 419-437 [Abstract] [Full Text]  
  • Abuladze, T., Li, M., Menetrez, M. Y., Dean, T., Senecal, A., Sulakvelidze, A. (2008). Bacteriophages Reduce Experimental Contamination of Hard Surfaces, Tomato, Spinach, Broccoli, and Ground Beef by Escherichia coli O157:H7. Appl. Environ. Microbiol. 74: 6230-6238 [Abstract] [Full Text]  
  • Ma, Y., Pacan, J. C., Wang, Q., Xu, Y., Huang, X., Korenevsky, A., Sabour, P. M. (2008). Microencapsulation of Bacteriophage Felix O1 into Chitosan-Alginate Microspheres for Oral Delivery. Appl. Environ. Microbiol. 74: 4799-4805 [Abstract] [Full Text]  
  • Stenholm, A. R., Dalsgaard, I., Middelboe, M. (2008). Isolation and Characterization of Bacteriophages Infecting the Fish Pathogen Flavobacterium psychrophilum. Appl. Environ. Microbiol. 74: 4070-4078 [Abstract] [Full Text]  
  • McLaughlin, M. R., Brooks, J. P. (2008). EPA Worst Case Water Microcosms for Testing Phage Biocontrol of Salmonella. J. Environ. Qual. 37: 266-271 [Abstract] [Full Text]  
  • Andreatti Filho, R. L., Higgins, J. P., Higgins, S. E., Gaona, G., Wolfenden, A. D., Tellez, G., Hargis, B. M. (2007). Ability of Bacteriophages Isolated from Different Sources to Reduce Salmonella enterica Serovar Enteritidis In Vitro and In Vivo. Poult. Sci. 86: 1904-1909 [Abstract] [Full Text]  
  • Anisimov, A. P., Amoako, K. K. (2006). Treatment of plague: promising alternatives to antibiotics.. J Med Microbiol 55: 1461-1475 [Abstract] [Full Text]  
  • Huff, W. E., Huff, G. R., Rath, N. C., Donoghue, A. M. (2006). Evaluation of the Influence of Bacteriophage Titer on the Treatment of Colibacillosis in Broiler Chickens. Poult. Sci. 85: 1373-1377 [Abstract] [Full Text]  
  • Coward, C., Grant, A. J., Swift, C., Philp, J., Towler, R., Heydarian, M., Frost, J. A., Maskell, D. J. (2006). Phase-Variable Surface Structures Are Required for Infection of Campylobacter jejuni by Bacteriophages.. Appl. Environ. Microbiol. 72: 4638-4647 [Abstract] [Full Text]  
  • McLaughlin, M. R., Balaa, M. F., Sims, J., King, R. (2006). Isolation of Salmonella Bacteriophages from Swine Effluent Lagoons. J. Environ. Qual. 35: 522-528 [Abstract] [Full Text]  
  • Loc Carrillo, C., Atterbury, R. J., El-Shibiny, A., Connerton, P. L., Dillon, E., Scott, A., Connerton, I. F. (2005). Bacteriophage Therapy To Reduce Campylobacter jejuni Colonization of Broiler Chickens. Appl. Environ. Microbiol. 71: 6554-6563 [Abstract] [Full Text]  
  • Atterbury, R. J., Dillon, E., Swift, C., Connerton, P. L., Frost, J. A., Dodd, C. E. R., Rees, C. E. D., Connerton, I. F. (2005). Correlation of Campylobacter Bacteriophage with Reduced Presence of Hosts in Broiler Chicken Ceca. Appl. Environ. Microbiol. 71: 4885-4887 [Abstract] [Full Text]  
  • Brussow, H. (2005). Phage therapy: the Escherichia coli experience. Microbiology 151: 2133-2140 [Abstract] [Full Text]  
  • Chibani-Chennoufi, S., Bruttin, A., Dillmann, M.-L., Brussow, H. (2004). Phage-Host Interaction: an Ecological Perspective. J. Bacteriol. 186: 3677-3686 [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»