This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nedelkov, D.
Right arrow Articles by Nelson, R. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nedelkov, D.
Right arrow Articles by Nelson, R. W.
Agricola
Right arrow Articles by Nedelkov, D.
Right arrow Articles by Nelson, R. W.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, September 2003, p. 5212-5215, Vol. 69, No. 9
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.9.5212-5215.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Detection of Staphylococcal Enterotoxin B via Biomolecular Interaction Analysis Mass Spectrometry

Dobrin Nedelkov* and Randall W. Nelson

Intrinsic Bioprobes Inc., Tempe, Arizona 85281

Received 4 February 2003/ Accepted 6 June 2003

Detection of Staphylococcus enterotoxin B (SEB) by biomolecular interaction analysis mass spectrometry (BIA/MS) is presented in this work. The BIA/MS experiments were based on a surface plasmon resonance (SPR) MS immunoassay that detects affinity-captured SEB both via SPR and by means of exact and direct mass measurement by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. Experiments were performed with standard samples and food samples to assess the BIA/MS limit of detection for SEB and to set the experimental parameters for proper quantitation. Single and double SPR referencing was performed to accurately estimate the amount of the bound toxin. Reproducible detection of 1 ng of SEB per ml, corresponding to affinity capture and MS analysis of ~500 amol of SEB, was readily achieved from both the standard and mushroom samples. A certain amount of SEB degradation was indicated by the signals in the mass spectra. The combination of MS with SPR-based methods of detection creates a unique approach capable of quantifying and qualitatively analyzing protein toxins from pathogenic organisms.

* Corresponding author. Mailing address: Intrinsic Bioprobes Inc., 625 S. Smith Rd., Suite 22, Tempe, AZ 85281. Phone: (480) 804-1778. Fax: (480) 804-0778. E-mail: dnedelkov{at}intrinsicbio.com.

Applied and Environmental Microbiology, September 2003, p. 5212-5215, Vol. 69, No. 9
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.9.5212-5215.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Schlosser, G., Kacer, P., Kuzma, M., Szilagyi, Z., Sorrentino, A., Manzo, C., Pizzano, R., Malorni, L., Pocsfalvi, G. (2007). Coupling Immunomagnetic Separation on Magnetic Beads with Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Detection of Staphylococcal Enterotoxin B. Appl. Environ. Microbiol. 73: 6945-6952 [Abstract] [Full Text]  
  • Cook, E., Wang, X., Robiou, N., Fries, B. C. (2007). Measurement of Staphylococcal Enterotoxin B in Serum and Culture Supernatant with a Capture Enzyme-Linked Immunosorbent Assay. CVI 14: 1094-1101 [Abstract] [Full Text]  
  • Sapsford, K. E., Taitt, C. R., Loo, N., Ligler, F. S. (2005). Biosensor Detection of Botulinum Toxoid A and Staphylococcal Enterotoxin B in Food. Appl. Environ. Microbiol. 71: 5590-5592 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»