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Applied and Environmental Microbiology, September 2003, p. 5243-5247, Vol. 69, No. 9
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.9.5243-5247.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Prevalence of Escherichia coli O157 in Cattle Feeds in Midwestern Feedlots

Charles C. Dodd,1 Michael W. Sanderson,1* Jan M. Sargeant,2 T. G. Nagaraja,3 Richard D. Oberst,2 Robert A. Smith,4 and D. Dee Griffin5

Department of Clinical Sciences,1 Food Animal Health and Management Center,2 Department of Diagnostic Medicine and Pathobiology, Kansas State University, Manhattan, Kansas 66506,3 Department of Veterinary Clinical Sciences, Oklahoma State University, Stillwater, Oklahoma 74078,4 Great Plains Veterinary Educational Center, University of Nebraska, Clay Center, Nebraska 689335

Received 15 January 2003/ Accepted 23 June 2003

Comparisons of enrichment methods (with or without antibiotics and with or without a preenrichment step) using gram-negative (GN) broth or tryptic soy broth (TSB) were conducted with feeds inoculated with Escherichia coli O157:H7. TSB was more sensitive than GN broth, and TSB with a preenrichment step followed by TSB with antibiotics was more sensitive than plain TSB enrichment, in detecting E. coli O157 in inoculated feeds. Feed samples were collected from feed bunks from 54 feedlots to determine the prevalence of E. coli O157 in cattle feeds. TSB preenrichment followed by TSB with antibiotics and the standard GN broth enrichment were used for each feed sample. All samples underwent immunomagnetic separation and were plated onto sorbitol MacConkey agar with cefixime and potassium tellurite. Identification of E. coli O157 was based on indole production, positive latex agglutination for O157 antigen, API 20E test strip results, PCR for the eaeA gene, and the presence of at least one Shiga toxin. E. coli O157 was detected in 52 of 504 feed samples (10.3%) by using GN broth enrichment and in 46 of 504 feed samples (9.1%) by using TSB followed by TSB supplemented with cefixime and vancomycin. E. coli O157 was detected in 75 of 504 feed bunk samples (14.9%) by one or both methods. There was no correlation between E. coli O157 prevalence and generic coliform counts in feeds. The prevalence of E. coli O157 in cattle feed warrants further studies to increase our knowledge of the on-farm ecology of E. coli O157 in order to develop strategies to prevent food-borne disease in humans.

* Corresponding author. Mailing address: Department of Clinical Sciences, Kansas State University, 103J Mosier Hall, Manhattan, KS 66506. Phone: (785) 532-5700. Fax: (785) 532-4989. E-mail: sandersn{at}vet.k-state.edu.

Applied and Environmental Microbiology, September 2003, p. 5243-5247, Vol. 69, No. 9
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.9.5243-5247.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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