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Applied and Environmental Microbiology, September 2003, p. 5336-5342, Vol. 69, No. 9
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.9.5336-5342.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Similarity of Tetracycline Resistance Genes Isolated from Fish Farm Bacteria to Those from Clinical Isolates

Manabu Furushita,1,2* Tsuneo Shiba,2 Toshimichi Maeda,2 Megumi Yahata,2 Azusa Kaneoka,2 Yukinori Takahashi,3 Keizo Torii,1 Tadao Hasegawa,1 and Michio Ohta1

Department of Bacteriology, Graduate School of Medicine, Nagoya University, Tsurumai, Showa-ku, Nagoya 466-8550,1 Department of Food Science and Technology,2 Department of Applied Aquabiology, National Fisheries University, Shimonoseki 759-6595, Japan3

Received 31 March 2003/ Accepted 7 July 2003

Tetracycline-resistant (Tetr) bacteria were isolated from fishes collected at three different fish farms in the southern part of Japan in August and September 2000. Of the 66 Tetr gram-negative strains, 29 were identified as carrying tetB only. Four carried tetY, and another four carried tetD. Three strains carried tetC, two strains carried tetB and tetY, and one strain carried tetC and tetG. Sequence analyses indicated the identity in Tetr genes between the fish farm bacteria and clinical bacteria: 99.3 to 99.9% for tetB, 98.2 to 100% for tetC, 99.7 to 100% for tetD, 92.0 to 96.2% for tetG, and 97.1 to 100% for tetY. Eleven of the Tetr strains transferred Tetr genes by conjugation to Escherichia coli HB-101. All transconjugants were resistant to tetracycline, oxycycline, doxycycline, and minocycline. The donors included strains of Photobacterium, Vibrio, Pseudomonas, Alteromonas, Citrobacter, and Salmonella spp., and they transferred tetB, tetY, or tetD to the recipients. Because NaCl enhanced their growth, these Tetr strains, except for the Pseudomonas, Citrobacter, and Salmonella strains, were recognized as marine bacteria. Our results suggest that tet genes from fish farm bacteria have the same origins as those from clinical strains.

* Corresponding author. Mailing address: Department of Food Science and Technology, National Fisheries University, Nagata-honmachi, Shimonoseki, Yamaguchi 759-6595, Japan. Phone: 81-832-86-5111. Fax: 81-832-86-7434. E-mail: furushita{at}fish-u.ac.jp.

Applied and Environmental Microbiology, September 2003, p. 5336-5342, Vol. 69, No. 9
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.9.5336-5342.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Brown, M. G., Mitchell, E. H., Balkwill, D. L. (2008). Tet 42, a Novel Tetracycline Resistance Determinant Isolated from Deep Terrestrial Subsurface Bacteria. Antimicrob. Agents Chemother. 52: 4518-4521 [Abstract] [Full Text]  
  • Lau, S. K. P., Wong, G. K. M., Li, M. W. S., Woo, P. C. Y., Yuen, K.-y. (2008). Distribution and molecular characterization of tetracycline resistance in Laribacter hongkongensis. J Antimicrob Chemother 61: 488-497 [Abstract] [Full Text]  
  • Furushita, M., Okamoto, A., Maeda, T., Ohta, M., Shiba, T. (2005). Isolation of Multidrug-Resistant Stenotrophomonas maltophilia from Cultured Yellowtail (Seriola quinqueradiata) from a Marine Fish Farm. Appl. Environ. Microbiol. 71: 5598-5600 [Abstract] [Full Text]  
  • Poole, K. (2005). Efflux-mediated antimicrobial resistance. J Antimicrob Chemother 56: 20-51 [Abstract] [Full Text]  


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