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Applied and Environmental Microbiology, September 2003, p. 5364-5371, Vol. 69, No. 9
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.9.5364-5371.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Morphological, Host Range, and Genetic Characterization of Two Coliphages

Lawrence Goodridge, Alicia Gallaccio, and Mansel W. Griffiths*

Canadian Research Institute for Food Safety, Department of Food Science, University of Guelph, Guelph, Ontario, Canada N1G 2W1

Received 20 November 2002/ Accepted 3 July 2003

Two coliphages, AR1 and LG1, were characterized based on their morphological, host range, and genetic properties. Transmission electron microscopy showed that both phages belonged to the Myoviridae; phage particles of LG1 were smaller than those of AR1 and had an isometric head 68 nm in diameter and a complex contractile tail 111 nm in length. Transmission electron micrographs of AR1 showed phage particles consisting of an elongated isometric head of 103 by 74 nm and a complex contractile tail 116 nm in length. Both phages were extensively tested on many strains of Escherichia coli and other enterobacteria. The results showed that both phages could infect many serotypes of E. coli. Among the enterobacteria, Proteus mirabilis, Shigella dysenteriae, and two Salmonella strains were lysed by the phages. The genetic material of AR1 and LG1 was characterized. Phage LG1 had a genome size of 49.5 kb compared to 150 kb for AR1. Restriction endonuclease analysis showed that several restriction enzymes could degrade DNA from both phages. The morphological, genome size, and restriction endonuclease similarities between AR1 and phage T4 were striking. Southern hybridizations showed that AR1 and T4 are genetically related. The wide host ranges of phages AR1 and LG1 suggest that they may be useful as biocontrol, therapeutic, or diagnostic agents to control and detect the prevalence of E. coli in animals and food.

* Corresponding author. Mailing address: Canadian Research Institute for Food Safety, Department of Food Science, University of Guelph, Guelph, Ontario, Canada N1G 2W1. Phone: (519) 824-4120. Fax: (519) 824-6631. E-mail: mgriffit{at}uoguelph.ca.

Applied and Environmental Microbiology, September 2003, p. 5364-5371, Vol. 69, No. 9
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.9.5364-5371.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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