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Applied and Environmental Microbiology, September 2003, p. 5410-5413, Vol. 69, No. 9
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.9.5410-5413.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

The Metabolic Pathway of 4-Aminophenol in Burkholderia sp. Strain AK-5 Differs from That of Aniline and Aniline with C-4 Substituents

Shinji Takenaka,1 Susumu Okugawa,2 Maho Kadowaki,1 Shuichiro Murakami,1 and Kenji Aoki1*

Department of Biofunctional Chemistry, Faculty of Agriculture,1 Division of Science of Biological Resources, Graduate School of Science and Technology, Kobe University, Rokko, Kobe, Japan2

Received 10 March 2003/ Accepted 2 July 2003

Burkholderia sp. strain AK-5 utilized 4-aminophenol as the sole carbon, nitrogen, and energy source. A pathway for the metabolism of 4-aminophenol in strain AK-5 was proposed based on the identification of three key metabolites by gas chromatography-mass spectrometry analysis. Strain AK-5 converted 4-aminophenol to 1,2,4-trihydroxybenzene via 1,4-benzenediol. 1,2,4-Trihydroxybenzene 1,2-dioxygenase cleaved the benzene ring of 1,2,4-trihydroxybenzene to form maleylacetic acid. The enzyme showed a high dioxygenase activity only for 1,2,4-trihydroxybenzene, with Km and Vmax values of 9.6 µM and 6.8 µmol min-1 mg of protein-1, respectively.

* Corresponding author. Mailing address: Department of Biofunctional Chemistry, Faculty of Agriculture, Kobe University, Rokko, Kobe 657-8501, Japan. Phone: 81 78 803 5891. Fax: 81 78 882 0481. E-mail: kaoki{at}kobe-u.ac.jp.

Applied and Environmental Microbiology, September 2003, p. 5410-5413, Vol. 69, No. 9
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.9.5410-5413.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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