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Applied and Environmental Microbiology, September 2003, p. 5585-5592, Vol. 69, No. 9
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.9.5585-5592.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

A Gene Cluster for Chlorate Metabolism in Ideonella dechloratans

Helena Danielsson Thorell, Katarina Stenklo, Jan Karlsson, and Thomas Nilsson*

Department of Chemistry, Karlstad University, SE 651 88 Karlstad, Sweden

Received 24 February 2003/ Accepted 29 June 2003

Chlorate reductase has been isolated from the chlorate-respiring bacterium Ideonella dechloratans, and the genes encoding the enzyme have been sequenced. The enzyme is composed of three different subunits and contains molybdopterin, iron, probably in iron-sulfur clusters, and heme b. The genes (clr) encoding chlorate reductase are arranged as clrABDC, where clrA, clrB, and clrC encode the subunits and clrD encodes a specific chaperone. Judging from the subunit composition, cofactor content, and sequence comparisons, chlorate reductase belongs to class II of the dimethyl sulfoxide reductase family. The clr genes are preceded by a novel insertion sequence (transposase gene surrounded by inverted repeats), denoted ISIde1. Further upstream, we find the previously characterized gene for chlorite dismutase (cld), oriented in the opposite direction. Chlorate metabolism in I. dechloratans starts with the reduction of chlorate, which is followed by the decomposition of the resulting chlorite to chloride and molecular oxygen. The present work reveals that the genes encoding the enzymes catalyzing both these reactions are in close proximity.

* Corresponding author. Mailing address: Department of Chemistry, Karlstad University, SE 651 88 Karlstad, Sweden. Phone: 46-54-7001776. Fax: 46-54-7001457. E-mail: thomas.nilsson{at}kau.se.

Applied and Environmental Microbiology, September 2003, p. 5585-5592, Vol. 69, No. 9
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.9.5585-5592.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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