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Applied and Environmental Microbiology, September 2003, p. 5690-5692, Vol. 69, No. 9
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.9.5690-5692.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Prevalence and Characteristics of eae-Positive Escherichia coli from Healthy Cattle in Japan

Hideki Kobayashi,^1* Akihiko Miura,² Hiroko Hayashi,¹ Torata Ogawa,³ Takayuki Endô,⁴ Eiji Hata,¹ Masashi Eguchi,¹ and Kôsi Yamamoto¹

National Institute of Animal Health, Tsukuba, Ibaraki 305-0856,¹ Nagasaki Chûô Livestock Hygiene Center, Nagasaki,² Fukuoka Chûô Livestock Hygiene Center, Fukuoka,³ Okitama Livestock Hygiene Center, Yamagata, Japan⁴

Received 26 February 2003/ Accepted 27 June 2003

The prevalence of eae-positive Escherichia coli (eaeEC) in Japan was examined using rectal stool samples taken from 35 calves less than 1 month old, 107 calves more than 1 to 3 months old, 88 heifers more than 3 to 6 months old, 214 heifers over 6 months old, and cows from 95 farms. Screening with eae PCR revealed the prevalence to be, with increasing age, 31.4, 8.4, 26.1, and 14.5%, respectively. Of 51 selected eaeEC strains, more than 40% were serotyped as O26, O103, O111, O145, or O157, which are frequently detected as enterohemorrhagic E. coli types. Four strains were identified as recently reported intimin types , , and .

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* Corresponding author. Mailing address: Clinical Epidemiology Section, Department of Epidemiology, National Institute of Animal Health, 3-1-5, Kannondai, Tsukuba 305-0856, Japan. Phone and fax: 81-29-838-7798. E-mail: reptile{at}affrc.go.jp.

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Applied and Environmental Microbiology, September 2003, p. 5690-5692, Vol. 69, No. 9
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.9.5690-5692.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

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