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Applied and Environmental Microbiology, September 2003, p. 5699-5701, Vol. 69, No. 9
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.9.5699-5701.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Phenotypic Changes Resulting from Distinct Point Mutations in the Azospirillum brasilense glnA Gene, Encoding Glutamine Synthetase

Anne Van Dommelen, Veerle Keijers, An Wollebrants, and Jozef Vanderleyden*

Center of Microbial and Plant Genetics, Katholieke Universiteit Leuven, B-3001 Heverlee, Belgium

Received 24 March 2003/ Accepted 18 June 2003

Sequencing the glnA genes of two chemically induced Azospirillum brasilense glutamine synthetase mutants revealed an Arg->Cys mutation, corresponding to the glutamate binding site, in one mutant and an Asp->Asn mutation, corresponding to the ammonium binding site, in the second mutant. The phenotypic changes in these mutants are discussed in relation to their genotypes.

* Corresponding author. Mailing address: Center of Microbial and Plant Genetics, K. U. Leuven, Kasteelpark Arenberg, 20, B-3001 Heverlee, Belgium. Phone: 32 16 32 96 79. Fax: 32 16 32 19 66. E-mail: Jozef.Vanderleyden{at}agr.kuleuven.ac.be.

Applied and Environmental Microbiology, September 2003, p. 5699-5701, Vol. 69, No. 9
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.9.5699-5701.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.





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