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Applied and Environmental Microbiology, September 2003, p. 5711-5715, Vol. 69, No. 9
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.9.5711-5715.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Role for Mannose-Sensitive Hemagglutinin in Promoting Interactions between Vibrio cholerae El Tor and Mussel Hemolymph

Massimiliano Zampini,1 Laura Canesi,2 Michele Betti,2 Caterina Ciacci,2 Renato Tarsi,1 Gabriella Gallo,3 and Carla Pruzzo1*

Istituto di Microbiologia e Scienze Biomediche, Università Politecnica delle Marche, Ancona,1 Istituto di Scienze Fisiologiche, Università di Urbino, Urbino,2 DIBISAA, Università di Genova, Genoa, Italy3

Received 24 February 2003/ Accepted 27 June 2003

The role of mannose-sensitive hemagglutinin (MSHA) in Vibrio cholerae O1 El Tor interactions with hemolymph of the mussel Mytilus galloprovincialis was studied. Bacterial adherence to and association with hemocytes were evaluated at 4 and 18°C, respectively. In hemolymph serum, the wild-type strain N16961 adhered to and associated with hemocytes about twofold more efficiently than its mutant lacking MSHA. In artificial seawater (ASW), no significant differences between the two strains were observed. N16961 was also more sensitive to hemocyte bactericidal activity than its MSHA mutant; in fact, the percentages of killed bacteria after 120 min of incubation were 60 and 34%, respectively. The addition of D-mannose abolished the serum-mediated increase in adherence, association, and sensitivity to killing of the wild-type strain without affecting the interactions of the mutant. A similar increase in N16961 adherence to hemocytes was observed when serum was adsorbed with MSHA-deficient bacteria. In contrast, serum adsorbed with either wild-type V. cholerae El Tor or wild-type Escherichia coli carrying type 1 fimbriae was no longer able to increase adherence of N16961 to hemocytes. The results indicate that hemolymph-soluble factors are involved in interactions between hemocytes and mannose-sensitive adhesins.

* Corresponding author. Mailing address: Istituto di Microbiologia e Scienze Biomediche, Università Politecnica delle Marche, Via Ranieri Monte d'Ago, 60131 Ancona, Italy. Phone: 39-071-220-4397. Fax: 39-071-220-4393. E-mail: c.pruzzo{at}unian.it.

Applied and Environmental Microbiology, September 2003, p. 5711-5715, Vol. 69, No. 9
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.9.5711-5715.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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