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Applied and Environmental Microbiology, September 2003, p. 5726-5730, Vol. 69, No. 9
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.9.5726-5730.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Molecular Detection and Quantitation of the Red Tide Dinoflagellate Karenia brevis in the Marine Environment

University of South Florida, College of Marine Science, St. Petersburg, Florida 33701¹

Received 7 March 2003/ Accepted 20 June 2003

A real-time reverse transcription-PCR method targeting the rbcL gene was developed for the detection and quantitation of the Florida red tide organism, Karenia brevis. The assay was sensitive to less than 1 cell per reaction, did not detect rbcL from 38 nontarget taxa, and accurately quantitated K. brevis organisms in red tide samples from around Florida. These studies have resulted in a sensitive and specific method for K. brevis detection in the marine environment.

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