This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow E-mail this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Richard, C.
Right arrow Articles by Prevost, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Richard, C.
Right arrow Articles by Prevost, H.
Agricola
Right arrow Articles by Richard, C.
Right arrow Articles by Prevost, H.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, January 2004, p. 248-254, Vol. 70, No. 1
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.1.248-254.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Generation and Utilization of Polyclonal Antibodies to a Synthetic C-Terminal Amino Acid Fragment of Divercin V41, a Class IIa Bacteriocin

Christelle Richard,1 Djamel Drider,1 Ismael Fliss,2 Sandra Denery,3 and Herve Prevost1*

Unité de Recherche sur la Qualité Microbiologique et Aromatique des Aliments (QM2A), ENITIAA, 44322 Nantes cedex 3,1 Unité de Recherche sur les Protéines Végétales et leurs Interactions, INRA, 44316 Nantes cedex 3, France,3 Dairy Research Centre STELA, Université Laval, Quebec, Canada G1K 7P42

Received 6 May 2003/ Accepted 8 October 2003

Polyclonal antibodies have been generated by immunization of rabbits with a chemically synthesized C-terminal part of divercin V41 (DvnCt) conjugated to the carrier protein keyhole limpet hemocyanin (KLH). The sensitivity and reactivity of the DvnCt-KLH-generated antibodies were evaluated by enzyme-linked immunosorbent assay (ELISA) using supernatant from cultures of 13 representative lactic acid bacterium strains, and specificity was confirmed by Western blot analysis. Anti-DvnCt-KLH antibodies were able to recognize not only divercin V41 but also enterocin P and piscicocin V1b, two other members of the class IIa bacteriocins. Production and activity of DvnV41 were evaluated by ELISA and activity tests during the growth of Carnobacterium divergens V41 in MRS medium containing or not containing Tween 80. Divercin V41, enterocin P, and piscicocin V1b were therefore purified by a single-step immunoaffinity chromatography method using a Sepharose matrix CNBr-activated directed binding of anti-DvnCt-KLH polyclonal antibodies.

* Corresponding author. Mailing address: Unité de Recherche sur la Qualité Microbiologique et Aromatique des Aliments (QM2A), ENITIAA, Rue de la Géraudière, BP 82225, 44322 Nantes cedex 3, France. Phone: 33 2 51785524. Fax: 33 2 51785520. E-mail: prevost{at}enitiaa-nantes.fr.

Applied and Environmental Microbiology, January 2004, p. 248-254, Vol. 70, No. 1
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.1.248-254.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Rihakova, J., Petit, V. W., Demnerova, K., Prevost, H., Rebuffat, S., Drider, D. (2009). Insights into Structure-Activity Relationships in the C-Terminal Region of Divercin V41, a Class IIa Bacteriocin with High-Level Antilisterial Activity. Appl. Environ. Microbiol. 75: 1811-1819 [Abstract] [Full Text]  
  • Criado, R., Gutierrez, J., Martin, M., Herranz, C., Hernandez, P. E., Cintas, L. M. (2006). Immunochemical Characterization of Temperature-Regulated Production of Enterocin L50 (EntL50A and EntL50B), Enterocin P, and Enterocin Q by Enterococcus faecium L50. Appl. Environ. Microbiol. 72: 7634-7643 [Abstract] [Full Text]  
  • Richard, C., Drider, D., Elmorjani, K., Marion, D., Prevost, H. (2004). Heterologous Expression and Purification of Active Divercin V41, a Class IIa Bacteriocin Encoded by a Synthetic Gene in Escherichia coli. J. Bacteriol. 186: 4276-4284 [Abstract] [Full Text]  


Copyright © 2010 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»