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Applied and Environmental Microbiology, January 2004, p. 280-284, Vol. 70, No. 1
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.1.280-284.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Characterization of a New erm-Related Macrolide Resistance Gene Present in Probiotic Strains of Bacillus clausii

Bülent Bozdogan,{dagger} Sébastien Galopin, and Roland Leclercq*

Service de Microbiologie, CHU Cote de Nacre, Caen, France

Received 4 September 2003/ Accepted 10 October 2003

The mechanism of resistance to macrolides, lincosamides, and streptogramins B was studied in four Bacillus clausii strains that are mixed in a probiotic administered to humans for prevention of gastrointestinal side effects due to oral antibiotic chemotherapy and in three reference strains of B. clausii, DSM8716, ATCC 21536, and ATCC 21537. An 846-bp gene called erm(34), which is related to the erm genes conferring resistance to these antibiotics by ribosomal methylation, was cloned from total DNA of B. clausii DSM8716 into Escherichia coli. The deduced amino acid sequence presented 61% identity with that of Erm(D) from B. licheniformis, B. halodurans, and B. anthracis. Pulsed-field gel electrophoresis of total DNA digested by I-CeuI, followed by hybridization with an erm(34)-specific probe, indicated a chromosomal location of the gene in all B. clausii strains. Repeated attempts to transfer resistance to macrolides by conjugation from B. clausii strains to Enterococcus faecalis JH2-2, E. faecium HM1070, and B. subtilis UCN19 were unsuccessful.

* Corresponding author. Mailing address: CHU de Caen Service de Microbiologie, CHU Cote de Nacre, Ave. Cote de Nacre, 14033 Caen Cedex, France. Phone: 33-231064572. Fax: 33-231064573. E-mail: leclercq-r{at}chu-caen.fr.

{dagger} Present address: Department of Pathology, Hershey Medical Center, Hershey, PA 17033.

Applied and Environmental Microbiology, January 2004, p. 280-284, Vol. 70, No. 1
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.1.280-284.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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