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Applied and Environmental Microbiology, January 2004, p. 346-355, Vol. 70, No. 1
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.1.346-355.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Frequency, Size, and Localization of Bacterial Aggregates on Bean Leaf Surfaces

J.-M. Monier and S. E. Lindow*

Department of Plant and Microbial Biology, University of California, Berkeley, California 94720

Received 28 April 2003/ Accepted 26 September 2003

Using epifluorescence microscopy and image analysis, we have quantitatively described the frequency, size, and spatial distribution of bacterial aggregates on leaf surfaces of greenhouse-grown bean plants inoculated with the plant-pathogenic bacterium Pseudomonas syringae pv. syringae strain B728a. Bacterial cells were not randomly distributed on the leaf surface but occurred in a wide range of cluster sizes, ranging from single cells to over 104 cells per aggregate. The average cluster size increased through time, and aggregates were more numerous and larger when plants were maintained under conditions of high relative humidity levels than under dry conditions. The large majority of aggregates observed were small (less than 100 cells), and aggregate sizes exhibited a strong right-hand-skewed frequency distribution. While large aggregates are not frequent on a given leaf, they often accounted for the majority of cells present. We observed that up to 50% of cells present on a leaf were located in aggregates containing 103 cells or more. Aggregates were associated with several different anatomical features of the leaf surface but not with stomates. Aggregates were preferentially associated with glandular trichomes and veins. The biological and ecological significance of aggregate formation by epiphytic bacteria is discussed.

* Corresponding author. Mailing address: Department of Plant and Microbial Biology, 111 Koshland Hall, University of California, Berkeley, CA 94720-3102. Phone: (510) 642-4174. Fax: (510) 642-4995. E-mail: icelab{at}socrates.berkeley.edu.

Applied and Environmental Microbiology, January 2004, p. 346-355, Vol. 70, No. 1
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.1.346-355.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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