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Applied and Environmental Microbiology, January 2004, p. 377-384, Vol. 70, No. 1
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.1.377-384.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Longitudinal Study of Fecal Shedding of Escherichia coli O157:H7 in Feedlot Cattle: Predominance and Persistence of Specific Clonal Types despite Massive Cattle Population Turnover

J. T. LeJeune,1* T. E. Besser,1 D. H. Rice,2 J. L. Berg,3 R. P. Stilborn,3 and D. D. Hancock2

Depertment of Veterinary Microbiology and Pathology,1 Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Washington State University, Pullman, Washington 99164,2 Lakeside Research, Brooks, Alberta T1R 1B7, Canada3

Received 25 July 2003/ Accepted 10 October 2003

Identification of the sources and methods of transmission of Escherichia coli O157:H7 in feedlot cattle may facilitate the development of on-farm control measures for this important food-borne pathogen. The prevalence of E. coli O157:H7 in fecal samples of commercial feedlot cattle in 20 feedlot pens between April and September 2000 was determined throughout the finishing feeding period prior to slaughter. Using immunomagnetic separation, E. coli O157:H7 was isolated from 636 of 4,790 (13%) fecal samples in this study, with highest prevalence earliest in the feeding period. No differences were observed in the fecal or water trough sediment prevalence values of E. coli O157:H7 in 10 pens supplied with chlorinated drinking water supplies compared with nonchlorinated water pens. Pulsed-field gel electrophoresis of XbaI-digested bacterial DNA of the 230 isolates obtained from eight of the pens revealed 56 unique restriction endonuclease digestion patterns (REDPs), although nearly 60% of the isolates belonged to a group of four closely related genetic subtypes that were present in each of the pens and throughout the sampling period. The other REDPs were typically transiently detected, often in single pens and on single sample dates, and in many cases were also closely related to the four predominant REDPs. The persistence and predominance of a few REDPs observed over the entire feeding period on this livestock operation highlight the importance of the farm environment, and not necessarily the incoming cattle, as a potential source or reservoir of E. coli O157:H7 on farms.

* Corresponding author. Present address: Food Animal Health Research Program, Ohio Agricultural Research and Development Center, Ohio State University, 1680 Madison Ave., Wooster, OH 44691. Phone: (330) 263-3739. Fax: (330) 263-3677. E-mail: lejeune.3{at}osu.edu.

Applied and Environmental Microbiology, January 2004, p. 377-384, Vol. 70, No. 1
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.1.377-384.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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