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Applied and Environmental Microbiology, January 2004, p. 452-458, Vol. 70, No. 1
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.1.452-458.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Phylogenetic Analysis of the Hypervariable Region of the 18S rRNA Gene of Cryptosporidium Oocysts in Feces of Canada Geese (Branta canadensis): Evidence for Five Novel Genotypes

Kristen L. Jellison,1* Daniel L. Distel,2 Harold F. Hemond,1 and David B. Schauer3,4

Department of Civil and Environmental Engineering,1 Biological Engineering Division,3 Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139,4 Department of Biochemistry, Microbiology, and Molecular Biology, University of Maine, Orono, Maine 044692

Received 20 June 2003/ Accepted 29 September 2003

To assess genetic diversity in Cryptosporidium oocysts from Canada geese, 161 fecal samples from Canada geese in the United States were analyzed. Eleven (6.8%) were positive for Cryptosporidium spp. following nested PCR amplification of the hypervariable region of the 18S rRNA gene. Nine PCR products from geese were cloned and sequenced, and all nine diverged from previously reported Cryptosporidium 18S rRNA gene sequences. Five sequences were very similar or identical to each other but genetically distinct from that of Cryptosporidium baileyi; two were most closely related to, but genetically distinct from, the first five; and two were distinct from any other sequence analyzed. One additional sequence in the hypervariable region of the 18S rRNA gene isolated from a cormorant was identical to that of C. baileyi. Phylogenetic analysis provided evidence for new genotypes of Cryptosporidium species in Canada geese. Results of this study suggest that the taxonomy of Cryptosporidium species in geese is complex and that a more complete understanding of genetic diversity among these parasites will facilitate our understanding of oocyst sources and species in the environment.

* Corresponding author. Mailing address: Lehigh University, Fritz Engineering Laboratory, 13 E. Packer Ave., Bethlehem, PA 18015. Phone: (610) 758-3555. Fax: (610) 758-6405. E-mail: kjellison{at}lehigh.edu.

Applied and Environmental Microbiology, January 2004, p. 452-458, Vol. 70, No. 1
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.1.452-458.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Ng, J., Pavlasek, I., Ryan, U. (2006). Identification of Novel Cryptosporidium Genotypes from Avian Hosts. Appl. Environ. Microbiol. 72: 7548-7553 [Abstract] [Full Text]  
  • Zhou, L., Kassa, H., Tischler, M. L., Xiao, L. (2004). Host-Adapted Cryptosporidium spp. in Canada Geese (Branta canadensis). Appl. Environ. Microbiol. 70: 4211-4215 [Abstract] [Full Text]  


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