This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Azevedo, N. F.
Right arrow Articles by Vieira, M. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Azevedo, N. F.
Right arrow Articles by Vieira, M. J.
Agricola
Right arrow Articles by Azevedo, N. F.
Right arrow Articles by Vieira, M. J.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, January 2004, p. 490-493, Vol. 70, No. 1
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.1.490-493.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Nutrient Shock and Incubation Atmosphere Influence Recovery of Culturable Helicobacter pylori from Water

N. F. Azevedo,1,2 A. P. Pacheco,1 C. W. Keevil,2 and M. J. Vieira1*

Centro de Engenharia Biológica, Universidade do Minho, 4700-057 Braga, Portugal,1 Environmental Healthcare Unit, School of Biological Sciences, University of Southampton, Southampton, United Kingdom2

Received 20 June 2003/ Accepted 16 October 2003

Three different media—Columbia agar, Wilkins-Chalgren agar, and Helicobacter pylori special peptone agar—were prepared in a diluted version and compared to the standard medium formulation in order to study a possible nutrient shock effect observed when recovering H. pylori from water by counting the number of CFU. This same parameter was subsequently used to evaluate the influence of the incubation atmosphere by using a modular atmosphere-controlled system to provide different atmospheres and by employing an established gas generation kit as a control. Both a low nutrient content of the media and a rapidly achieved microaerophilic incubation atmosphere proved to increase the numbers of environment-stressed H. pylori organisms recovered. An atmosphere of 5% CO2, 5% O2, and 3% H2 is recommended, although other atmospheres with a low oxygen concentration are also acceptable. Besides highlighting and assessing the importance of several factors in the culturability of H. pylori, this paper demonstrates the potential ability to develop an optimized technique for recovery of this pathogen from water.

* Corresponding author. Mailing address: Centro de Engenharia Biologica, Universidade do Minho, 4700-057 Braga, Portugal. Phone: 351-253-604404. Fax: 351-253-678986. E-mail: mjv{at}deb.uminho.pt.

Applied and Environmental Microbiology, January 2004, p. 490-493, Vol. 70, No. 1
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.1.490-493.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Giao, M. S., Azevedo, N. F., Wilks, S. A., Vieira, M. J., Keevil, C. W. (2008). Persistence of Helicobacter pylori in Heterotrophic Drinking-Water Biofilms. Appl. Environ. Microbiol. 74: 5898-5904 [Abstract] [Full Text]  
  • Azevedo, N. F., Almeida, C., Fernandes, I., Cerqueira, L., Dias, S., Keevil, C. W., Vieira, M. J. (2008). Survival of Gastric and Enterohepatic Helicobacter spp. in Water: Implications for Transmission. Appl. Environ. Microbiol. 74: 1805-1811 [Abstract] [Full Text]  
  • Griffin, D. W. (2007). Atmospheric Movement of Microorganisms in Clouds of Desert Dust and Implications for Human Health. Clin. Microbiol. Rev. 20: 459-477 [Abstract] [Full Text]  
  • Azevedo, N. F., Almeida, C., Cerqueira, L., Dias, S., Keevil, C. W., Vieira, M. J. (2007). Coccoid Form of Helicobacter pylori as a Morphological Manifestation of Cell Adaptation to the Environment. Appl. Environ. Microbiol. 73: 3423-3427 [Abstract] [Full Text]  
  • Azevedo, N. F., Pinto, A. R., Reis, N. M., Vieira, M. J., Keevil, C. W. (2006). Shear Stress, Temperature, and Inoculation Concentration Influence the Adhesion of Water-Stressed Helicobacter pylori to Stainless Steel 304 and Polypropylene. Appl. Environ. Microbiol. 72: 2936-2941 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»