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Applied and Environmental Microbiology, January 2004, p. 518-526, Vol. 70, No. 1
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.1.518-526.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

In Vitro and In Vivo Inhibition of Helicobacter pylori by Lactobacillus casei Strain Shirota

D. Sgouras,1,{dagger} P. Maragkoudakis,2,{dagger} K. Petraki,3,{dagger} B. Martinez-Gonzalez,1 E. Eriotou,1 S. Michopoulos,4 G. Kalantzopoulos,2 E. Tsakalidou,2 and A. Mentis1*

Laboratory of Medical Microbiology, Hellenic Pasteur Institute,1 Laboratory of Dairy Research, Agricultural University of Athens,2 Laboratory of Pathology, Hippocration Hospital,3 Gastroenterology Clinic, Alexandra Hospital, Athens, Greece4

Received 2 April 2003/ Accepted 20 October 2003

We studied the potential inhibitory effect of Lactobacillus casei strain Shirota (from the fermented milk product Yakult [Yakult Ltd., Tokyo, Japan]) on Helicobacter pylori by using (i) in vitro inhibition assays with H. pylori SS1 (Sydney strain 1) and nine H. pylori clinical isolates and (ii) the in vivo H. pylori SS1 mouse model of infection over a period of 9 months. In vitro activity against H. pylori SS1 and all of the clinical isolates was observed in the presence of viable L. casei strain Shirota cells but not in the cell-free culture supernatant, although there was profound inhibition of urease activity. In vivo experiments were performed by oral administration of L. casei strain Shirota in the water supply over a period of 9 months to 6-week-old C57BL/6 mice previously infected with H. pylori SS1 (study group; n = 25). Appropriate control groups of H. pylori-infected but untreated animals (n = 25) and uninfected animals given L. casei strain Shirota (n = 25) also were included in the study. H. pylori colonization and development of gastritis were assessed at 1, 2, 3, 6, and 9 months postinfection. A significant reduction in the levels of H. pylori colonization was observed in the antrum and body mucosa in vivo in the lactobacillus-treated study group, as assessed by viable cultures, compared to the levels in the H. pylori-infected control group. This reduction was accompanied by a significant decline in the associated chronic and active gastric mucosal inflammation observed at each time point throughout the observation period. A trend toward a decrease in the anti-H. pylori immunoglobulin G response was measured in the serum of the animals treated with lactobacillus, although this decrease was not significant.

* Corresponding author. Mailing address: Laboratory of Medical Microbiology, Hellenic Pasteur Institute, Vas. Sophias 127, 115 21 Athens, Greece. Phone: 30210-6478816. Fax: 30210-6423498. E-mail: mentis{at}mail.pasteur.gr.

{dagger} D.S., P.M., and K.P. contributed equally to this work.

Applied and Environmental Microbiology, January 2004, p. 518-526, Vol. 70, No. 1
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.1.518-526.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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