This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sgouras, D.
Right arrow Articles by Mentis, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sgouras, D.
Right arrow Articles by Mentis, A.
Agricola
Right arrow Articles by Sgouras, D.
Right arrow Articles by Mentis, A.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, January 2004, p. 518-526, Vol. 70, No. 1
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.1.518-526.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

In Vitro and In Vivo Inhibition of Helicobacter pylori by Lactobacillus casei Strain Shirota

D. Sgouras,1,{dagger} P. Maragkoudakis,2,{dagger} K. Petraki,3,{dagger} B. Martinez-Gonzalez,1 E. Eriotou,1 S. Michopoulos,4 G. Kalantzopoulos,2 E. Tsakalidou,2 and A. Mentis1*

Laboratory of Medical Microbiology, Hellenic Pasteur Institute,1 Laboratory of Dairy Research, Agricultural University of Athens,2 Laboratory of Pathology, Hippocration Hospital,3 Gastroenterology Clinic, Alexandra Hospital, Athens, Greece4

Received 2 April 2003/ Accepted 20 October 2003

We studied the potential inhibitory effect of Lactobacillus casei strain Shirota (from the fermented milk product Yakult [Yakult Ltd., Tokyo, Japan]) on Helicobacter pylori by using (i) in vitro inhibition assays with H. pylori SS1 (Sydney strain 1) and nine H. pylori clinical isolates and (ii) the in vivo H. pylori SS1 mouse model of infection over a period of 9 months. In vitro activity against H. pylori SS1 and all of the clinical isolates was observed in the presence of viable L. casei strain Shirota cells but not in the cell-free culture supernatant, although there was profound inhibition of urease activity. In vivo experiments were performed by oral administration of L. casei strain Shirota in the water supply over a period of 9 months to 6-week-old C57BL/6 mice previously infected with H. pylori SS1 (study group; n = 25). Appropriate control groups of H. pylori-infected but untreated animals (n = 25) and uninfected animals given L. casei strain Shirota (n = 25) also were included in the study. H. pylori colonization and development of gastritis were assessed at 1, 2, 3, 6, and 9 months postinfection. A significant reduction in the levels of H. pylori colonization was observed in the antrum and body mucosa in vivo in the lactobacillus-treated study group, as assessed by viable cultures, compared to the levels in the H. pylori-infected control group. This reduction was accompanied by a significant decline in the associated chronic and active gastric mucosal inflammation observed at each time point throughout the observation period. A trend toward a decrease in the anti-H. pylori immunoglobulin G response was measured in the serum of the animals treated with lactobacillus, although this decrease was not significant.

* Corresponding author. Mailing address: Laboratory of Medical Microbiology, Hellenic Pasteur Institute, Vas. Sophias 127, 115 21 Athens, Greece. Phone: 30210-6478816. Fax: 30210-6423498. E-mail: mentis{at}mail.pasteur.gr.

{dagger} D.S., P.M., and K.P. contributed equally to this work.

Applied and Environmental Microbiology, January 2004, p. 518-526, Vol. 70, No. 1
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.1.518-526.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Watanabe, T., Nishio, H., Tanigawa, T., Yamagami, H., Okazaki, H., Watanabe, K., Tominaga, K., Fujiwara, Y., Oshitani, N., Asahara, T., Nomoto, K., Higuchi, K., Takeuchi, K., Arakawa, T. (2009). Probiotic Lactobacillus casei strain Shirota prevents indomethacin-induced small intestinal injury: involvement of lactic acid. Am. J. Physiol. Gastrointest. Liver Physiol. 297: G506-G513 [Abstract] [Full Text]  
  • Rodriguez, C., Medici, M., Rodriguez, A. V., Mozzi, F., Font de Valdez, G. (2009). Prevention of chronic gastritis by fermented milks made with exopolysaccharide-producing Streptococcus thermophilus strains. J DAIRY SCI 92: 2423-2434 [Abstract] [Full Text]  
  • Myllyluoma, E., Ahonen, A.-M., Korpela, R., Vapaatalo, H., Kankuri, E. (2008). Effects of Multispecies Probiotic Combination on Helicobacter pylori Infection In Vitro. CVI 15: 1472-1482 [Abstract] [Full Text]  
  • Miki, K., Urita, Y., Ishikawa, F., Iino, T., Shibahara-Sone, H., Akahoshi, R., Mizusawa, S., Nose, A., Nozaki, D., Hirano, K., Nonaka, C., Yokokura, T. (2007). Effect of Bifidobacterium bifidum Fermented Milk on Helicobacter pylori and Serum Pepsinogen Levels in Humans. J DAIRY SCI 90: 2630-2640 [Abstract] [Full Text]  
  • Lesbros-Pantoflickova, D., Corthesy-Theulaz, I., Blum, A. L. (2007). Helicobacter pylori and Probiotics. J. Nutr. 137: 812S-818S [Abstract] [Full Text]  
  • Paraschos, S., Magiatis, P., Mitakou, S., Petraki, K., Kalliaropoulos, A., Maragkoudakis, P., Mentis, A., Sgouras, D., Skaltsounis, A.-L. (2007). In Vitro and In Vivo Activities of Chios Mastic Gum Extracts and Constituents against Helicobacter pylori. Antimicrob. Agents Chemother. 51: 551-559 [Abstract] [Full Text]  
  • Sgouras, D. N., Panayotopoulou, E. G., Martinez-Gonzalez, B., Petraki, K., Michopoulos, S., Mentis, A. (2005). Lactobacillus johnsonii La1 Attenuates Helicobacter pylori-Associated Gastritis and Reduces Levels of Proinflammatory Chemokines in C57BL/6 Mice. CVI 12: 1378-1386 [Abstract] [Full Text]  
  • Fayol-Messaoudi, D., Berger, C. N., Coconnier-Polter, M.-H., Lievin-Le Moal, V., Servin, A. L. (2005). pH-, Lactic Acid-, and Non-Lactic Acid-Dependent Activities of Probiotic Lactobacilli against Salmonella enterica Serovar Typhimurium. Appl. Environ. Microbiol. 71: 6008-6013 [Abstract] [Full Text]  
  • Horie, K., Horie, N., Abdou, A. M., Yang, J.-O., Yun, S.-S., Chun, H.-N., Park, C.-K., Kim, M., Hatta, H. (2004). Suppressive Effect of Functional Drinking Yogurt Containing Specific Egg Yolk Immunoglobulin on Helicobacter pylori in Humans. J DAIRY SCI 87: 4073-4079 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»