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Applied and Environmental Microbiology, January 2004, p. 599-602, Vol. 70, No. 1
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.1.599-602.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Analysis of the gyrA Gene of Clinical Yersinia ruckeri Isolates with Reduced Susceptibility to Quinolones

Alicia Gibello, M. Concepción Porrero, M. Mar Blanco, Ana I. Vela, Pilar Liébana, Miguel A. Moreno, José F. Fernández-Garayzábal,* and Lucas Domínguez

Departamento Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain

Received 9 June 2003/ Accepted 2 October 2003

Antimicrobial susceptibility of seven clinical strains of Yersinia ruckeri representative of those isolated between 1994 and 2002 from a fish farm with endemic enteric redmouth disease was studied. All isolates displayed indistinguishable pulsed-field gel electrophoresis restriction patterns, indicating that they represented a single strain. However, considering both inhibition zone diameters (IZD) and MICs, the isolates recovered in 2001-2002 formed a separate cluster with lower levels of susceptibility to all the quinolones tested, especially nalidixic acid (NA) and oxolinic acid (OA), compared with the isolates recovered between 1994 and 1998. Analysis of the PCR product of the quinolone resistance-determining region of the gyrA gene from clinical isolates of Y. ruckeri with reduced susceptibility to OA and NA revealed a single amino acid substitution, Ser-83 to Arg-83 (Escherichia coli numbering). Identical substitution was observed in induced OA-resistant mutant strains, which displayed IZD and MICs of quinolones similar to those of the clinical isolates of Y. ruckeri with reduced susceptibility to these antimicrobial agents. These data indicate in that for Y. ruckeri, the substitution of Ser by Arg at position 83 of the gyrA gene is associated with reduced susceptibility to quinolones.

* Corresponding author. Mailing address: Dpto. Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain. Phone: 34 91 3943716. Fax: 34 91 3943908. E-mail: garayzab{at}vet.ucm.es.

Applied and Environmental Microbiology, January 2004, p. 599-602, Vol. 70, No. 1
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.1.599-602.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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