This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bertin, Y.
Right arrow Articles by Martin, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bertin, Y.
Right arrow Articles by Martin, C.
Agricola
Right arrow Articles by Bertin, Y.
Right arrow Articles by Martin, C.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, January 2004, p. 61-68, Vol. 70, No. 1
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.1.61-68.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Localization of the Insertion Site and Pathotype Determination of the Locus of Enterocyte Effacement of Shiga Toxin-Producing Escherichia coli Strains

Yolande Bertin,1 Karima Boukhors,1 Valerie Livrelli,2 and Christine Martin1*

Laboratoire de Microbiologie, Centre de Recherche INRA de Clermont-Ferrand-Theix, 63122 St-Genès Chapanelle,1 Groupe de Recherche Pathogénie Bactérienne Intestinale, Faculté de Pharmacie, Université d'Auvergne Clermont 1, 63001 Clermont-Ferrand Cedex, France2

Received 15 July 2003/ Accepted 10 October 2003

Of 220 Shiga toxin-producing Escherichia coli (STEC) strains collected in central France from healthy cattle, food samples, and asymptomatic children, 12 possessed the eae gene included in the locus of enterocyte effacement (LEE) pathogenicity island. Based on gene typing, we observed 7 different eae espA espB tir pathotypes among the 12 STEC strains and described the new espAßv variant. As previously observed, the O157 serogroup is associated with eae{gamma}, O26 is associated with eaeß, and O103 is associated with eae{varepsilon}. However, the unexpected eae{zeta} allele was detected in 5 of the 12 isolates. PCR amplification and pulsed-field gel electrophoresis using the I-CeuI endonuclease followed by Southern hybridization indicated that the LEE was inserted in the vicinity of the selC (three isolates), pheU (two isolates), or pheV (six isolates) tRNA gene. Six isolates harbored two or three of these tRNA loci altered by the insertion of integrase genes (CP4-int and/or int-phe), suggesting the insertion of additional foreign DNA fragments at these sites. In spite of great genetic diversity of LEE pathotypes and LEE insertion sites, bovine strains harbor alleles of LEE genes that are frequently found in clinical STEC strains isolated from outbreaks and sporadic cases around the world, underscoring the potential risk of the bovine strains on human health.

* Corresponding author. Mailing address: Laboratoire de Microbiologie, INRA de Clermont-Ferrand-Theix, 63122 St-Genès Champanelle, France. Phone: (33) 4 73 62 42 47. Fax: (33) 4 73 63 45 81. E-mail: cmartin{at}clermont.inra.fr.

Applied and Environmental Microbiology, January 2004, p. 61-68, Vol. 70, No. 1
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.1.61-68.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Geue, L., Klare, S., Schnick, C., Mintel, B., Meyer, K., Conraths, F. J. (2009). Analysis of the Clonal Relationship of Serotype O26:H11 Enterohemorrhagic Escherichia coli Isolates from Cattle. Appl. Environ. Microbiol. 75: 6947-6953 [Abstract] [Full Text]  
  • Muller, D., Benz, I., Liebchen, A., Gallitz, I., Karch, H., Schmidt, M. A. (2009). Comparative Analysis of the Locus of Enterocyte Effacement and Its Flanking Regions. Infect. Immun. 77: 3501-3513 [Abstract] [Full Text]  
  • Vidal, M., Prado, V., Whitlock, G. C., Solari, A., Torres, A. G., Vidal, R. M. (2008). Subtractive hybridization and identification of putative adhesins in a Shiga toxin-producing eae-negative Escherichia coli. Microbiology 154: 3639-3648 [Abstract] [Full Text]  
  • Muniesa, M., Schembri, M. A., Hauf, N., Chakraborty, T. (2006). Active Genetic Elements Present in the Locus of Enterocyte Effacement in Escherichia coli O26 and Their Role in Mobility. Infect. Immun. 74: 4190-4199 [Abstract] [Full Text]  
  • Loukiadis, E., Kerouredan, M., Beutin, L., Oswald, E., Brugere, H. (2006). Characterization of Shiga Toxin Gene (stx)-Positive and Intimin Gene (eae)-Positive Escherichia coli Isolates from Wastewater of Slaughterhouses in France.. Appl. Environ. Microbiol. 72: 3245-3251 [Abstract] [Full Text]  
  • Geue, L., Selhorst, T., Schnick, C., Mintel, B., Conraths, F. J. (2006). Analysis of the Clonal Relationship of Shiga Toxin-Producing Escherichia coli Serogroup O165:H25 Isolated from Cattle.. Appl. Environ. Microbiol. 72: 2254-2259 [Abstract] [Full Text]  
  • Gilmour, M. W., Tracz, D. M., Andrysiak, A. K., Clark, C. G., Tyson, S., Severini, A., Ng, L.-K. (2006). Use of the espZ Gene Encoded in the Locus of Enterocyte Effacement for Molecular Typing of Shiga Toxin-Producing Escherichia coli. J. Clin. Microbiol. 44: 449-458 [Abstract] [Full Text]  
  • Girardeau, J. P., Dalmasso, A., Bertin, Y., Ducrot, C., Bord, S., Livrelli, V., Vernozy-Rozand, C., Martin, C. (2005). Association of Virulence Genotype with Phylogenetic Background in Comparison to Different Seropathotypes of Shiga Toxin-Producing Escherichia coli Isolates. J. Clin. Microbiol. 43: 6098-6107 [Abstract] [Full Text]  
  • Vernozy-Rozand, C., Montet, M. P., Bertin, Y., Trably, F., Girardeau, J. P., Martin, C., Livrelli, V., Beutin, L. (2004). Serotyping, stx2 Subtyping, and Characterization of the Locus of Enterocyte Effacement Island of Shiga Toxin-Producing Escherichia coli and E. coli O157:H7 Strains Isolated from the Environment in France. Appl. Environ. Microbiol. 70: 2556-2559 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»