AEM
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cléry-Barraud, C.
Right arrow Articles by Vidal, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cléry-Barraud, C.
Right arrow Articles by Vidal, D.
Agricola
Right arrow Articles by Cléry-Barraud, C.
Right arrow Articles by Vidal, D.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, January 2004, p. 635-637, Vol. 70, No. 1
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.1.635-637.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Combined Effects of High Hydrostatic Pressure and Temperature for Inactivation of Bacillus anthracis Spores

Cécile Cléry-Barraud,1 Agnès Gaubert,2 Patrick Masson,1 and Dominique Vidal2*

Département de Biologie des Agents Transmissibles,2 Département de Toxicologie, Centre de Recherches du Service de Santé des Armées, 38702 La Tronche Cedex, France1

Received 2 May 2003/ Accepted 10 October 2003

Spores of Bacillus anthracis are known to be extremely resistant to heat treatment, irradiation, desiccation, and disinfectants. To determine inactivation kinetics of spores by high pressure, B. anthracis spores of a Sterne strain-derived mutant deficient in the production of the toxin components (strain RP42) were exposed to pressures ranging from 280 to 500 MPa for 10 min to 6 h, combined with temperatures ranging from 20 to 75°C. The combination of heat and pressure resulted in complete destruction of B. anthracis spores, with a D value (exposure time for 90% inactivation of the spore population) of approximately 4 min after pressurization at 500 MPa and 75°C, compared to 160 min at 500 MPa and 20°C and 348 min at atmospheric pressure (0.1 MPa) and 75°C. The use of high pressure for spore inactivation represents a considerable improvement over other available methods of spore inactivation and could be of interest for antigenic spore preparation.

* Corresponding author. Mailing address: Département de Biologie des Agents Transmissibles, CRSSA, BP 87, F-38702 La Tronche Cédex, France. Phone: 33 (0) 4 76 63 69 19. Fax: 33 (0) 4 76 63 69 17. E-mail: domvidal{at}crssa.net.

Applied and Environmental Microbiology, January 2004, p. 635-637, Vol. 70, No. 1
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.1.635-637.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. Microbiol. Mol. Biol. Rev. Eukaryot. Cell All ASM Journals

Copyright © 2004 by the American Society for Microbiology. All rights reserved.