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Applied and Environmental Microbiology, January 2004, p. 638-641, Vol. 70, No. 1
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.1.638-641.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Role of Aminotransferase IlvE in Production of Branched-Chain Fatty Acids by Lactococcus lactis subsp. lactis{dagger}

Balasubramanian Ganesan and Bart C. Weimer*

Western Dairy Center, Center for Microbe Detection and Physiology, Center for Integrated BioSystems, and Department of Nutrition and Food Sciences, Utah State University, Logan, Utah 84322-8700

Received 4 August 2003/ Accepted 15 October 2003

The objective of this study was to determine the role of a lactococcal branched-chain amino acid aminotransferase gene, ilvE, in the production of branched-chain fatty acids. Lactococcus lactis subsp. lactis LM0230 and an ilvE deletion mutant, JLS450, produced branched-chain fatty acids from amino and {alpha}-keto acids at levels above {alpha}-keto acid spontaneous degradation and the fatty acids' flavor thresholds. The deletion mutant produced the same amounts of branched-chain fatty acids from precursor amino acids as did the parent. This was not the case, however, for the production of branched-chain fatty acids from the corresponding precursor {alpha}-keto acids. The deletion mutant produced a set of fatty acids different from that produced by the parent. We concluded from these observations that ilvE plays a role in the specific type of fatty acids produced but has little influence on the total amount of fatty acids produced by lactococci.

* Corresponding author. Mailing address: Center for Integrated BioSystems, Utah State University, Logan, UT 84322-4700. Phone: (435) 797-2753. Fax: (435) 797-2357. E-mail: bcweimer{at}cc.usu.edu.

{dagger} Contribution number 7537 of the Utah Agricultural Experiment Station.

Applied and Environmental Microbiology, January 2004, p. 638-641, Vol. 70, No. 1
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.1.638-641.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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