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Applied and Environmental Microbiology, January 2004, p. 638-641, Vol. 70, No. 1
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.1.638-641.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Role of Aminotransferase IlvE in Production of Branched-Chain Fatty Acids by Lactococcus lactis subsp. lactis

Western Dairy Center, Center for Microbe Detection and Physiology, Center for Integrated BioSystems, and Department of Nutrition and Food Sciences, Utah State University, Logan, Utah 84322-8700

Received 4 August 2003/ Accepted 15 October 2003

The objective of this study was to determine the role of a lactococcal branched-chain amino acid aminotransferase gene, ilvE, in the production of branched-chain fatty acids. Lactococcus lactis subsp. lactis LM0230 and an ilvE deletion mutant, JLS450, produced branched-chain fatty acids from amino and -keto acids at levels above -keto acid spontaneous degradation and the fatty acids' flavor thresholds. The deletion mutant produced the same amounts of branched-chain fatty acids from precursor amino acids as did the parent. This was not the case, however, for the production of branched-chain fatty acids from the corresponding precursor -keto acids. The deletion mutant produced a set of fatty acids different from that produced by the parent. We concluded from these observations that ilvE plays a role in the specific type of fatty acids produced but has little influence on the total amount of fatty acids produced by lactococci.

Contribution number 7537 of the Utah Agricultural Experiment Station.

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