This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gray, M. J.
Right arrow Articles by Wiedmann, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gray, M. J.
Right arrow Articles by Wiedmann, M.
Agricola
Right arrow Articles by Gray, M. J.
Right arrow Articles by Wiedmann, M.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, October 2004, p. 5833-5841, Vol. 70, No. 10
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.10.5833-5841.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Listeria monocytogenes Isolates from Foods and Humans Form Distinct but Overlapping Populations

Michael J. Gray,1 Ruth N. Zadoks,1 Esther D. Fortes,1 Belgin Dogan,1 Steven Cai,1,{dagger} Yuhuan Chen,2 Virginia N. Scott,2 David E. Gombas,2 Kathryn J. Boor,1 and Martin Wiedmann1*

Department of Food Science, Cornell University, Ithaca, New York,1 National Food Processors Association, Washington, D.C.2

Received 21 March 2004/ Accepted 21 June 2004

A total of 502 Listeria monocytogenes isolates from food and 492 from humans were subtyped by EcoRI ribotyping and PCR-restriction fragment length polymorphism analysis of the virulence gene hly. Isolates were further classified into genetic lineages based on subtyping results. Food isolates were obtained through a survey of selected ready-to-eat food products in Maryland and California in 2000 and 2001. Human isolates comprised 42 isolates from invasive listeriosis cases reported in Maryland and California during 2000 and 2001 as well as an additional 450 isolates from cases that had occurred throughout the United States, predominantly from 1997 to 2001. Assignment of isolates to lineages and to the majority of L. monocytogenes subtypes was significantly associated with the isolate source (food or human), although most subtypes and lineages included both human and food isolates. Some subtypes were also significantly associated with isolation from specific food types. Tissue culture plaque assay characterization of the 42 human isolates from Maryland and California and of 91 representative food isolates revealed significantly higher average infectivity and cell-to-cell spread for the human isolates, further supporting the hypothesis that food and human isolates form distinct populations. Combined analysis of subtype and cytopathogenicity data showed that strains classified into specific ribotypes previously linked to multiple human listeriosis outbreaks, as well as those classified into lineage I, are more common among human cases and generate larger plaques than other subtypes, suggesting that these subtypes may represent particularly virulent clonal groups. These data will provide a framework for prediction of the public health risk associated with specific L. monocytogenes subtypes.

* Corresponding author. Mailing address: Department of Food Science, 412B Stocking Hall, Cornell University, Ithaca, NY 14853. Phone: (607) 254-2838. Fax: (607) 254-4868. E-mail: mw16{at}cornell.edu.

{dagger} Present address: School of Medicine and Biomedical Sciences, State University of New York, Buffalo, New York.

Applied and Environmental Microbiology, October 2004, p. 5833-5841, Vol. 70, No. 10
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.10.5833-5841.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Roig-Sagues, A. X., Velazquez, R. M., Montealegre-Agramont, P., Lopez-Pedemonte, T. J., Brinez-Zambrano, W. J., Guamis-Lopez, B., Hernandez-Herrero, M. M. (2009). Fat content increases the lethality of ultra-high-pressure homogenization on Listeria monocytogenes in milk. J DAIRY SCI 92: 5396-5402 [Abstract] [Full Text]  
  • Roberts, A. J., Williams, S. K., Wiedmann, M., Nightingale, K. K. (2009). Some Listeria monocytogenes Outbreak Strains Demonstrate Significantly Reduced Invasion, inlA Transcript Levels, and Swarming Motility In Vitro. Appl. Environ. Microbiol. 75: 5647-5658 [Abstract] [Full Text]  
  • Donaldson, J. R., Nanduri, B., Burgess, S. C., Lawrence, M. L. (2009). Comparative Proteomic Analysis of Listeria monocytogenes Strains F2365 and EGD. Appl. Environ. Microbiol. 75: 366-373 [Abstract] [Full Text]  
  • Ward, T. J., Ducey, T. F., Usgaard, T., Dunn, K. A., Bielawski, J. P. (2008). Multilocus Genotyping Assays for Single Nucleotide Polymorphism-Based Subtyping of Listeria monocytogenes Isolates. Appl. Environ. Microbiol. 74: 7629-7642 [Abstract] [Full Text]  
  • Van Stelten, A., Nightingale, K. K. (2008). Development and Implementation of a Multiplex Single-Nucleotide Polymorphism Genotyping Assay for Detection of Virulence-Attenuating Mutations in the Listeria monocytogenes Virulence-Associated Gene inlA. Appl. Environ. Microbiol. 74: 7365-7375 [Abstract] [Full Text]  
  • Dumas, E., Meunier, B., Berdague, J.-L., Chambon, C., Desvaux, M., Hebraud, M. (2008). Comparative Analysis of Extracellular and Intracellular Proteomes of Listeria monocytogenes Strains Reveals a Correlation between Protein Expression and Serovar. Appl. Environ. Microbiol. 74: 7399-7409 [Abstract] [Full Text]  
  • Nightingale, K. K., Ivy, R. A., Ho, A. J., Fortes, E. D., Njaa, B. L., Peters, R. M., Wiedmann, M. (2008). inlA Premature Stop Codons Are Common among Listeria monocytogenes Isolates from Foods and Yield Virulence-Attenuated Strains That Confer Protection against Fully Virulent Strains. Appl. Environ. Microbiol. 74: 6570-6583 [Abstract] [Full Text]  
  • Orsi, R. H., Ripoll, D. R., Yeung, M., Nightingale, K. K., Wiedmann, M. (2007). Recombination and positive selection contribute to evolution of Listeria monocytogenes inlA. Microbiology 153: 2666-2678 [Abstract] [Full Text]  
  • Felicio, M. T. S., Hogg, T., Gibbs, P., Teixeira, P., Wiedmann, M. (2007). Recurrent and Sporadic Listeria monocytogenes Contamination in Alheiras Represents Considerable Diversity, Including Virulence-Attenuated Isolates. Appl. Environ. Microbiol. 73: 3887-3895 [Abstract] [Full Text]  
  • Ho, A. J., Lappi, V. R., Wiedmann, M. (2007). Longitudinal Monitoring of Listeria monocytogenes Contamination Patterns in a Farmstead Dairy Processing Facility. J DAIRY SCI 90: 2517-2524 [Abstract] [Full Text]  
  • Fugett, E. B., Schoonmaker-Bopp, D., Dumas, N. B., Corby, J., Wiedmann, M. (2007). Pulsed-Field Gel Electrophoresis (PFGE) Analysis of Temporally Matched Listeria monocytogenes Isolates from Human Clinical Cases, Foods, Ruminant Farms, and Urban and Natural Environments Reveals Source-Associated as Well as Widely Distributed PFGE Types. J. Clin. Microbiol. 45: 865-873 [Abstract] [Full Text]  
  • Chen, Y., Zhang, W., Knabel, S. J. (2007). Multi-Virulence-Locus Sequence Typing Identifies Single Nucleotide Polymorphisms Which Differentiate Epidemic Clones and Outbreak Strains of Listeria monocytogenes. J. Clin. Microbiol. 45: 835-846 [Abstract] [Full Text]  
  • Ducey, T. F., Page, B., Usgaard, T., Borucki, M. K., Pupedis, K., Ward, T. J. (2007). A Single-Nucleotide-Polymorphism-Based Multilocus Genotyping Assay for Subtyping Lineage I Isolates of Listeria monocytogenes. Appl. Environ. Microbiol. 73: 133-147 [Abstract] [Full Text]  
  • Kongo, J. M., Malcata, F. X., Ho, A. J., Wiedmann, M. (2006). Detection and characterization of Listeria monocytogenes in Sao Jorge (Portugal) cheese production.. J DAIRY SCI 89: 4456-4461 [Abstract] [Full Text]  
  • Nightingale, K. K., Lyles, K., Ayodele, M., Jalan, P., Nielsen, R., Wiedmann, M. (2006). Novel Method To Identify Source-Associated Phylogenetic Clustering Shows that Listeria monocytogenes Includes Niche-Adapted Clonal Groups with Distinct Ecological Preferences.. J. Clin. Microbiol. 44: 3742-3751 [Abstract] [Full Text]  
  • Liu, D. (2006). Identification, subtyping and virulence determination of Listeria monocytogenes, an important foodborne pathogen. J Med Microbiol 55: 645-659 [Abstract] [Full Text]  
  • Roberts, A., Nightingale, K., Jeffers, G., Fortes, E., Kongo, J. M., Wiedmann, M. (2006). Genetic and phenotypic characterization of Listeria monocytogenes lineage III.. Microbiology 152: 685-693 [Abstract] [Full Text]  
  • Gilbreth, S. E., Call, J. E., Wallace, F. M., Scott, V. N., Chen, Y., Luchansky, J. B. (2005). Relatedness of Listeria monocytogenes Isolates Recovered from Selected Ready-To-Eat Foods and Listeriosis Patients in the United States. Appl. Environ. Microbiol. 71: 8115-8122 [Abstract] [Full Text]  
  • Nightingale, K. K., Windham, K., Martin, K. E., Yeung, M., Wiedmann, M. (2005). Select Listeria monocytogenes Subtypes Commonly Found in Foods Carry Distinct Nonsense Mutations in inlA, Leading to Expression of Truncated and Secreted Internalin A, and Are Associated with a Reduced Invasion Phenotype for Human Intestinal Epithelial Cells. Appl. Environ. Microbiol. 71: 8764-8772 [Abstract] [Full Text]  
  • Zhang, C., Nietfeldt, J., Zhang, M., Benson, A. K. (2005). Functional Consequences of Genome Evolution in Listeria monocytogenes: the lmo0423 and lmo0422 Genes Encode {sigma}C and LstR, a Lineage II-Specific Heat Shock System. J. Bacteriol. 187: 7243-7253 [Abstract] [Full Text]  
  • Nightingale, K. K., Windham, K., Wiedmann, M. (2005). Evolution and Molecular Phylogeny of Listeria monocytogenes Isolated from Human and Animal Listeriosis Cases and Foods. J. Bacteriol. 187: 5537-5551 [Abstract] [Full Text]  
  • Roberts, A., Chan, Y., Wiedmann, M. (2005). Definition of Genetically Distinct Attenuation Mechanisms in Naturally Virulence-Attenuated Listeria monocytogenes by Comparative Cell Culture and Molecular Characterization. Appl. Environ. Microbiol. 71: 3900-3910 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»