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Applied and Environmental Microbiology, October 2004, p. 5859-5867, Vol. 70, No. 10
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.10.5859-5867.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Inheritance of Resistance to Bacillus thuringiensis subsp. kurstaki in Trichoplusia ni

Alida F. Janmaat,1* Ping Wang,2* Wendy Kain,2 Jian-Zhou Zhao,2 and Judith Myers1

Department of Zoology, University of British Columbia, Vancouver, British Columbia, Canada,1 Department of Entomology, New York State Agricultural Experiment Station, Cornell University, Geneva, New York2

Received 10 March 2004/ Accepted 4 June 2004

The genetic inheritance of resistance to a commercial formulation of Bacillus thuringiensis subsp. kurstaki was examined in a Trichoplusia ni colony initiated from a resistant population present in a commercial vegetable greenhouse in British Columbia, Canada. Progeny of F1 reciprocal crosses and backcrosses between F1 larvae and resistant (PR) and susceptible (PS) populations were assayed at different B. thuringiensis subsp. kurstaki concentrations. The responses of progeny of reciprocal F1 crosses were identical, indicating that the resistant trait was autosomal. The 50% lethal concentration for the F1 larvae was slightly higher than that for PS, suggesting that resistance is partially recessive. The responses of both backcross progeny (F1 x PR, F1 x PS) did not correspond to predictions from a single-locus model. The inclusion of a nonhomozygous resistant parental line in the monogenic model significantly increased the correspondence between the expected and observed results for the F1 x PR backcross but decreased the correspondence with the F1 x PS backcross results. This finding suggests that resistance to B. thuringiensis subsp. kurstaki in this T. ni population is due to more than one gene.

* Corresponding author. Mailing address for A.F.J.: Department of Zoology, University of British Columbia, 6270 University Blvd., Vancouver, British Columbia, Canada V6T 1Z4. Phone: (604) 822-5979. Fax: (604) 822-2416. E-mail: janmaat{at}zoology.ubc.ca. Mailing address for P.W.: Department of Entomology, Cornell University, New York State Agricultural Experiment Station, Geneva, NY 14456. Phone: (315) 787-2348. Fax: (315) 787-2326. E-mail: pw15{at}cornell.edu.

Applied and Environmental Microbiology, October 2004, p. 5859-5867, Vol. 70, No. 10
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.10.5859-5867.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Swiecicka, I., Bideshi, D. K., Federici, B. A. (2008). Novel Isolate of Bacillus thuringiensis subsp. thuringiensis That Produces a Quasicuboidal Crystal of Cry1Ab21 Toxic to Larvae of Trichoplusia ni. Appl. Environ. Microbiol. 74: 923-930 [Abstract] [Full Text]  


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