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Applied and Environmental Microbiology, October 2004, p. 6247-6256, Vol. 70, No. 10
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.10.6247-6256.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Comparative Analysis of Physical Maps of Four Bacillus subtilis (natto) Genomes

Dongru Qiu,1 Kyoko Fujita,2 Yuko Sakuma,2 Teruo Tanaka,3 Yoshiaki Ohashi,1 Hideyuki Ohshima,1 Masaru Tomita,1,4 and Mitsuhiro Itaya1,2*

Institute for Advanced Biosciences and Bioinformatics Program, Graduate School of Governance and Media, Keio University, Tsuruoka, Yamagata,1 Mitsubishi Kagaku Institute of Life Sciences, Machida, Tokyo,2 Department of Marine Science and Technology, Tokai University, Shizuoka,3 Department of Environmental Information, Keio University, Fujisawa, Kanagawa, Japan4

Received 23 December 2003/ Accepted 10 June 2004

The complete SfiI and I-CeuI physical maps of four Bacillus subtilis (natto) strains, which were previously isolated as natto (fermented soybean) starters, were constructed to elucidate the genome structure. Not only the similarity in genome size and organization but also the microheterogeneity of the gene context was revealed. No large-scale genome rearrangements among the four strains were indicated by mapping of the genes, including 10 rRNA operons (rrn) and relevant genes required for natto production, to the loci corresponding to those of the B. subtilis strain Marburg 168. However, restriction fragment length polymorphism and the presence or absence of strain-specific DNA sequences, such as the prophages SPß, skin element, and PBSX, as well as the insertion element IS4Bsu1, could be used to identify one of these strains as a Marburg type and the other three strains as natto types. The genome structure and gene heterogeneity were also consistent with the type of indigenous plasmids harbored by the strains.

* Corresponding author. Mailing address: Institute for Advanced Biosciences, Keio University, 403-1 Nipponkoku, Daihoji, Tsuruoka, Yamagata 997-0017, Japan. Phone: 81-235-29-0526. Fax: 81-235-29-0529. E-mail: mita2001{at}sfc.keio.ac.jp.

Applied and Environmental Microbiology, October 2004, p. 6247-6256, Vol. 70, No. 10
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.10.6247-6256.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Takahashi, K., Sekine, Y., Chibazakura, T., Yoshikawa, H. (2007). Development of an intermolecular transposition assay system in Bacillus subtilis 168 using IS4Bsu1 from Bacillus subtilis (natto). Microbiology 153: 2553-2559 [Abstract] [Full Text]  
  • Sakaya, N., Kaneko, S., Matsunaga, S., Itaya, M. (2006). Experimental Basis for a Stable Plasmid, pLS30, to Shuttle between Bacillus subtilis Species by Conjugational Transfer.. J Biochem 139: 557-561 [Abstract] [Full Text]  
  • Itaya, M., Tsuge, K., Koizumi, M., Fujita, K. (2005). From The Cover: Combining two genomes in one cell: Stable cloning of the Synechocystis PCC6803 genome in the Bacillus subtilis 168 genome. Proc. Natl. Acad. Sci. USA 102: 15971-15976 [Abstract] [Full Text]  


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