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Applied and Environmental Microbiology, October 2004, p. 6320-6323, Vol. 70, No. 10
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.10.6320-6323.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Repressor Mutant Forms of the Azospirillum brasilense NtrC Protein

Luciano F. Huergo, Marcelo C. Assumpção, Emanuel M. Souza, M. Berenice R. Steffens, M. Geoffrey Yates, Leda S. Chubatsu, and Fábio O. Pedrosa*

Department of Biochemistry and Molecular Biology, Universidade Federal do Paraná, Curitiba, Paraná, Brazil

Received 19 February 2004/ Accepted 10 June 2004

ABSTRACT

The Azospirillum brasilense mutant strains FP8 and FP9, after treatment with nitrosoguanidine, showed a null Nif phenotype and were unable to use nitrate as their sole nitrogen source. Sequencing of the ntrC genes revealed single nucleotide mutations in the NtrC nucleotide-binding site. The phenotypes of these strains are discussed in relation to their genotypes.

* Corresponding author. Mailing address: Department of Biochemistry and Molecular Biology, Universidade Federal do Paraná, CP 19046, 81531-990, Curitiba, Paraná, Brazil. Phone: 55 41 366 4398. Fax: 55 41 266 2042. E-mail: fpedrosa{at}ufpr.br.

Applied and Environmental Microbiology, October 2004, p. 6320-6323, Vol. 70, No. 10
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.10.6320-6323.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.





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