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Applied and Environmental Microbiology, October 2004, p. 6324-6328, Vol. 70, No. 10
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.10.6324-6328.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

A Single Amino Acid Substitution Converts {gamma}-Glutamyltranspeptidase to a Class IV Cephalosporin Acylase (Glutaryl-7-Aminocephalosporanic Acid Acylase)

Hideyuki Suzuki,* Chinatsu Miwa, Sayaka Ishihara, and Hidehiko Kumagai{dagger}

Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University, Kitashirakawa, Sakyo-ku, Kyoto, Japan

Received 28 January 2004/ Accepted 11 June 2004

ABSTRACT

The aspartyl residue at position 433 of {gamma}-glutamyltranspeptidase of Escherichia coli K-12 was replaced by an asparaginyl residue. This substitution enabled {gamma}-glutamyltranspeptidase to deacylate glutaryl-7-aminocephalosporanic acid, producing 7-aminocephalosporanic acid, which is a starting material for the synthesis of semisynthetic cephalosporins.

* Corresponding author. Mailing address: Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University, Oiwake-cho, Kitashirakawa, Sakyo-ku, Kyoto 606-8502, Japan. Phone: 81-75-753-6278. Fax: 81-75-753-6275. E-mail: hideyuki{at}lif.kyoto-u.ac.jp.

FOOTNOTES

{dagger} Present address: Research Institute of Agricultural Resources, Ishikawa Agricultural College, Ishikawa-gun, Ishikawa 921-8836, Japan.

Applied and Environmental Microbiology, October 2004, p. 6324-6328, Vol. 70, No. 10
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.10.6324-6328.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Yamada, C., Kijima, K., Ishihara, S., Miwa, C., Wada, K., Okada, T., Fukuyama, K., Kumagai, H., Suzuki, H. (2008). Improvement of the Glutaryl-7-Aminocephalosporanic Acid Acylase Activity of a Bacterial {gamma}-Glutamyltranspeptidase. Appl. Environ. Microbiol. 74: 3400-3409 [Abstract] [Full Text]  


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