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Applied and Environmental Microbiology, October 2004, p. 6329-6332, Vol. 70, No. 10
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.10.6329-6332.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Reverse Transcription-Booster PCR for Detection of Noroviruses in Shellfish

Dario De Medici,1* Luciana Croci,1 Elisabetta Suffredini,1 and Laura Toti1

Laboratorio Alimenti, Istituto Superiore di Sanità, Rome, Italy1

Received 10 November 2003/ Accepted 12 June 2004

ABSTRACT

The methods commonly used for norovirus (NV) detection are based on reverse transcription-PCR (RT-PCR) followed by confirmation of the amplified sequence. To increase sensitivity, an RT-booster PCR was developed. The proposed method showed an increase in sensitivity at least 2 log units for all the NV strains tested compared with the standard RT-PCR method. Higher sensitivity was confirmed in tests on experimentally and naturally contaminated shellfish.

* Corresponding author. Mailing address: Istituto Superiore di Sanità, Laboratorio Alimenti, Viale Regina Elena 299, 00161 Rome, Italy. Phone: 39 06 49902477. Fax: 39 06 49902045. E-mail dario.demedici{at}iss.it.

Applied and Environmental Microbiology, October 2004, p. 6329-6332, Vol. 70, No. 10
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.10.6329-6332.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Le Guyader, F. S., Bon, F., DeMedici, D., Parnaudeau, S., Bertone, A., Crudeli, S., Doyle, A., Zidane, M., Suffredini, E., Kohli, E., Maddalo, F., Monini, M., Gallay, A., Pommepuy, M., Pothier, P., Ruggeri, F. M. (2006). Detection of multiple noroviruses associated with an international gastroenteritis outbreak linked to oyster consumption.. J. Clin. Microbiol. 44: 3878-3882 [Abstract] [Full Text]  


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