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Applied and Environmental Microbiology, October 2004, p. 6337-6341, Vol. 70, No. 10
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.10.6337-6341.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Cloning and Expression of clt Genes Encoding Milk-Clotting Proteases from Myxococcus xanthus 422

M. Poza,1 M. Prieto-Alcedo,1 C. Sieiro,2 and T. G. Villa1*

Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Santiago de Compostela, Santiago de Compostela,1 Department of Microbiology, Faculty of Sciences, University of Vigo, Vigo, Spain2

Received 29 January 2004/ Accepted 20 June 2004

The screening of a gene library of the milk-clotting strain Myxococcus xanthus 422 constructed in Escherichia coli allowed the description of eight positive clones containing 26 open reading frames. Only three of them (cltA, cltB, and cltC) encoded proteins that exhibited intracellular milk-clotting ability in E. coli, Saccharomyces cerevisiae, and Pichia pastoris expression systems.

* Corresponding author. Mailing address: Department of Microbiology and Parasitology, Faculty of Pharmacy, Campus Sur 15782, Santiago de Compostela, Spain. Phone and fax: 34 981 592490. E-mail: mpvilla{at}usc.es.

Applied and Environmental Microbiology, October 2004, p. 6337-6341, Vol. 70, No. 10
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.10.6337-6341.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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