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Applied and Environmental Microbiology, October 2004, p. 6342-6346, Vol. 70, No. 10
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.10.6342-6346.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Isolation of Lightning-Competent Soil Bacteria

Hélène Cérémonie,1 François Buret,2 Pascal Simonet,1 and Timothy M. Vogel1*

Écologie Microbienne UMR-CNRS, Université Claude Bernard Lyon 1, Villeurbanne,1 Centre de Génie Electrique de Lyon, UPRESA CNRS, Ecole Centrale de Lyon, Ecully, France2

Received 27 February 2004/ Accepted 22 June 2004

ABSTRACT

Artificial transformation is typically performed in the laboratory by using either a chemical (CaCl2) or an electrical (electroporation) method. However, laboratory-scale lightning has been shown recently to electrotransform Escherichia coli strain DH10B in soil. In this paper, we report on the isolation of two "lightning-competent" soil bacteria after direct electroporation of the Nycodenz bacterial ring extracted from prairie soil in the presence of the pBHCRec plasmid (Tcr, Spr, Smr). The electrotransformability of the isolated bacteria was measured both in vitro (by electroporation cuvette) and in situ (by lightning in soil microcosm) and then compared to those of E. coli DH10B and Pseudomonas fluorescens C7R12. The electrotransformation frequencies measured reached 10–3 to 10–4 by electroporation and 10–4 to 10–5 by simulated lightning, while no transformation was observed in the absence of electrical current. Two of the isolated lightning-competent soil bacteria were identified as Pseudomonas sp. strains.

* Corresponding author. Mailing address: Écologie Microbienne UMR-CNRS 5557, Université Claude Bernard Lyon 1, BÂt. G. Mendel, 43 Blvd. du 11 Novembre 1918, 69622 Villeurbanne Cedex, France. Phone: (33) 4-72-43-27-58. Fax: (33) 4-72-43-12-23. E-mail: vogel{at}univ-lyon1.fr.

Applied and Environmental Microbiology, October 2004, p. 6342-6346, Vol. 70, No. 10
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.10.6342-6346.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Chee-Sanford, J. C., Mackie, R. I., Koike, S., Krapac, I. G., Lin, Y.-F., Yannarell, A. C., Maxwell, S., Aminov, R. I. (2009). Fate and Transport of Antibiotic Residues and Antibiotic Resistance Genes following Land Application of Manure Waste. J. Environ. Qual. 38: 1086-1108 [Abstract] [Full Text]  
  • Ceremonie, H., Buret, F., Simonet, P., Vogel, T. M. (2006). Natural Electrotransformation of Lightning-Competent Pseudomonas sp. Strain N3 in Artificial Soil Microcosms. Appl. Environ. Microbiol. 72: 2385-2389 [Abstract] [Full Text]  


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