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 Previous Article

Applied and Environmental Microbiology, October 2004, p. 6347-6351, Vol. 70, No. 10
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.10.6347-6351.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Genetic Identification of a Putative Vinyl Chloride Reductase in Dehalococcoides sp. Strain BAV1

Rosa Krajmalnik-Brown,1 Tina Hölscher,2 Ivy N. Thomson,3 F. Michael Saunders,1 Kirsti M. Ritalahti,1 and Frank E. Löffler1,3*

School of Civil and Environmental Engineering,1 School of Biology, Georgia Institute of Technology, Atlanta, Georgia,3 Fachgebiet Technische Biochemie, Institut für Biotechnologie, Technische Universität Berlin, Berlin, Germany2

Received 14 April 2004/ Accepted 2 June 2004

ABSTRACT

Dehalococcoides sp. strain BAV1 couples growth with the reductive dechlorination of vinyl chloride (VC) to ethene. Degenerate primers targeting conserved regions in reductive dehalogenase (RDase) genes were designed and used to PCR amplify putative RDase genes from strain BAV1. Seven unique RDase gene fragments were identified. Transcription analysis of VC-grown BAV1 cultures suggested that bvcA was involved in VC reductive dechlorination, and the complete sequence of bvcA was obtained. bvcA was absent in Dehalococcoides isolates that failed to respire VC, yet was detected in four of eight VC-respiring mixed cultures.

* Corresponding author. Mailing address: School of Civil and Environmental Engineering, 311 Ferst Dr., 3228 ES&T Building, Georgia Institute of Technology, Atlanta, GA 30332-0512. Phone: (404) 894-0279. Fax: (404) 894-8266. E-mail: frank.loeffler{at}ce.gatech.edu.

Applied and Environmental Microbiology, October 2004, p. 6347-6351, Vol. 70, No. 10
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.10.6347-6351.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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