This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Castro, H.
Right arrow Articles by Reddy, K. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Castro, H.
Right arrow Articles by Reddy, K. R.
Agricola
Right arrow Articles by Castro, H.
Right arrow Articles by Reddy, K. R.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, November 2004, p. 6559-6568, Vol. 70, No. 11
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.11.6559-6568.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Phylogenetic Characterization of Methanogenic Assemblages in Eutrophic and Oligotrophic Areas of the Florida Everglades{dagger}

Hector Castro,1 Andrew Ogram,1,2* and K. R. Reddy1

Soil and Water Science Department,1 Microbiology and Cell Science Department, University of Florida, Gainesville, Florida2

Received 4 May 2004/ Accepted 9 July 2004

Agricultural activities have produced well-documented changes in the Florida Everglades, including establishment of a gradient in phosphorus concentrations in Water Conservation Area 2A (WCA-2A) of the northern Everglades. An effect of increased phosphorus concentrations is increased methanogenesis in the eutrophic regions compared to the oligotrophic regions of WCA-2A. The goal of this study was to identify relationships between eutrophication and composition and activity of methanogenic assemblages in WCA-2A soils. Distributions of two genes associated with methanogens were characterized in soils taken from WCA-2A: the archaeal 16S rRNA gene and the methyl coenzyme M reductase gene. The richness of methanogen phylotypes was greater in eutrophic than in oligotrophic sites, and sequences related to previously cultivated and uncultivated methanogens were found. A preferential selection for the order Methanomicrobiales was observed in mcrA clone libraries, suggesting primer bias for this group. A greater diversity within the Methanomicrobiales was observed in mcrA clone libraries than in 16S rRNA gene libraries. 16S rRNA phylogenetic analyses revealed a dominance of clones related to Methanosaeta spp., an acetoclastic methanogen dominant in environments with low acetate concentrations. A significant number of clones were related to Methanomicrobiales, an order characterized by species utilizing hydrogen and formate as methanogenic substrates. No representatives of the orders Methanobacteriales and Methanococcales were found in any 16S rRNA clone library, although some Methanobacteriales were found in mcrA libraries. Hydrogenotrophs are the dominant methanogens in WCA-2A, and acetoclastic methanogen genotypes that proliferate in low acetate concentrations outnumber those that typically dominate in higher acetate concentrations.

* Corresponding author. Mailing address: P.O. Box 110290, University of Florida, Gainesville, FL 32611-0290. Phone: (352) 392-1951. Fax: (352) 392-3902. E-mail: avo{at}mail.ifas.ufl.edu.

{dagger} Florida Agricultural Experimental Station journal series no. R-10329.

Applied and Environmental Microbiology, November 2004, p. 6559-6568, Vol. 70, No. 11
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.11.6559-6568.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Steinberg, L. M., Regan, J. M. (2009). mcrA-Targeted Real-Time Quantitative PCR Method To Examine Methanogen Communities. Appl. Environ. Microbiol. 75: 4435-4442 [Abstract] [Full Text]  
  • Hartman, W. H., Richardson, C. J., Vilgalys, R., Bruland, G. L. (2008). Environmental and anthropogenic controls over bacterial communities in wetland soils. Proc. Natl. Acad. Sci. USA 105: 17842-17847 [Abstract] [Full Text]  
  • Steinberg, L. M., Regan, J. M. (2008). Phylogenetic Comparison of the Methanogenic Communities from an Acidic, Oligotrophic Fen and an Anaerobic Digester Treating Municipal Wastewater Sludge. Appl. Environ. Microbiol. 74: 6663-6671 [Abstract] [Full Text]  
  • Smith, J. M., Castro, H., Ogram, A. (2007). Structure and Function of Methanogens along a Short-Term Restoration Chronosequence in the Florida Everglades. Appl. Environ. Microbiol. 73: 4135-4141 [Abstract] [Full Text]  
  • Chauhan, A., Ogram, A. (2006). Phylogeny of Acetate-Utilizing Microorganisms in Soils along a Nutrient Gradient in the Florida Everglades.. Appl. Environ. Microbiol. 72: 6837-6840 [Abstract] [Full Text]  
  • Jiang, H., Dong, H., Zhang, G., Yu, B., Chapman, L. R., Fields, M. W. (2006). Microbial diversity in water and sediment of lake chaka, an athalassohaline lake in northwestern china.. Appl. Environ. Microbiol. 72: 3832-3845 [Abstract] [Full Text]  
  • Chauhan, A., Ogram, A. (2006). Fatty Acid-Oxidizing Consortia along a Nutrient Gradient in the Florida Everglades. Appl. Environ. Microbiol. 72: 2400-2406 [Abstract] [Full Text]  
  • Castro, H., Newman, S., Reddy, K. R., Ogram, A. (2005). Distribution and Stability of Sulfate-Reducing Prokaryotic and Hydrogenotrophic Methanogenic Assemblages in Nutrient-Impacted Regions of the Florida Everglades. Appl. Environ. Microbiol. 71: 2695-2704 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»