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Applied and Environmental Microbiology, November 2004, p. 6748-6752, Vol. 70, No. 11
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.11.6748-6752.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Seasonal Shedding of Multiple Cryptosporidium Genotypes in California Ground Squirrels (Spermophilus beecheyi)

Edward R. Atwill,1* Ralph Phillips,2 Maria Das Graças C. Pereira,1 Xunde Li,1 and Brenda McCowan1

Veterinary Medicine Teaching and Research Center, School of Veterinary Medicine, University of California—Davis, Tulare,1 University of California Cooperative Extension, Bakersfield, California2

Received 27 February 2004/ Accepted 16 July 2004

Twelve percent of 853 California ground squirrels (Spermophilus beecheyi) from six different geographic locations in Kern County, Calif., were found to be shedding on average 44,482 oocysts g of feces–1. The mean annual environmental loading rate of Cryptosporidium oocysts was 57,882 oocysts squirrel–1 day–1, with seasonal patterns of fecal shedding ranging from <10,000 oocysts squirrel–1 day–1 in fall, winter, and spring to levels of 2 x 105 oocysts squirrel–1 day–1 in summer. Juveniles were about twice as likely as adult squirrels to be infected and shed higher concentrations of oocysts than adults did, with particularly high levels of infection and shedding being found among juvenile male squirrels. Based on DNA sequencing of a portion of the 18S small-subunit rRNA gene, there existed three genotypes of Cryptosporidium species in these populations of squirrels (Sbey03a, Sbey03b, and Sbey03c; accession numbers AY462231 to AY462233, respectively). These unique DNA sequences were most closely related (96 to 97% homology) to porcine C. parvum (AF115377) and C. wrairi (AF115378). Inoculating BALB/c neonatal mice with up to 10,000 Sbey03b or Sbey03c fresh oocysts from different infected hosts did not produce detectable levels of infection, suggesting that this common genotype shed by California ground squirrels is not infectious for mice and may constitute a new species of Cryptosporidium.

* Corresponding author. Mailing address: Veterinary Medicine Teaching and Research Center, 18830 Road 112, Tulare, CA 93274. Phone: (559) 688-1731. Fax: (559) 686-4231. E-mail: ratwill{at}vmtrc.ucdavis.edu.

Applied and Environmental Microbiology, November 2004, p. 6748-6752, Vol. 70, No. 11
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.11.6748-6752.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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