This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pinhassi, J.
Right arrow Articles by Marrasé, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pinhassi, J.
Right arrow Articles by Marrasé, C.
Agricola
Right arrow Articles by Pinhassi, J.
Right arrow Articles by Marrasé, C.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, November 2004, p. 6753-6766, Vol. 70, No. 11
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.11.6753-6766.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Changes in Bacterioplankton Composition under Different Phytoplankton Regimens

Jarone Pinhassi,1* Maria Montserrat Sala,1 Harry Havskum,2 Francesc Peters,1 Òscar Guadayol,1 Andrea Malits,1,{ddagger} and Cèlia Marrasé1

Institut de Ciències del Mar-CMIMA (CSIC), Barcelona, Catalunya, Spain,1 Marine Biological Laboratory, University of Copenhagen, Helsingør, Denmark2

Received 13 May 2004/ Accepted 20 July 2004

The results of empirical studies have revealed links between phytoplankton and bacterioplankton, such as the frequent correlation between chlorophyll a and bulk bacterial abundance and production. Nevertheless, little is known about possible links at the level of specific taxonomic groups. To investigate this issue, seawater microcosm experiments were performed in the northwestern Mediterranean Sea. Turbulence was used as a noninvasive means to induce phytoplankton blooms dominated by different algae. Microcosms exposed to turbulence became dominated by diatoms, while small phytoflagellates gained importance under still conditions. Denaturing gradient gel electrophoresis (DGGE) of 16S rRNA gene fragments showed that changes in phytoplankton community composition were followed by shifts in bacterioplankton community composition, both as changes in the presence or absence of distinct bacterial phylotypes and as differences in the relative abundance of ubiquitous phylotypes. Sequencing of DGGE bands showed that four Roseobacter phylotypes were present in all microcosms. The microcosms with a higher proportion of phytoflagellates were characterized by four phylotypes of the Bacteroidetes phylum: two affiliated with the family Cryomorphaceae and two with the family Flavobacteriaceae. Two other Flavobacteriaceae phylotypes were characteristic of the diatom-dominated microcosms, together with one Alphaproteobacteria phylotype (Roseobacter) and one Gammaproteobacteria phylotype (Methylophaga). Phylogenetic analyses of published Bacteroidetes 16S rRNA gene sequences confirmed that members of the Flavobacteriaceae are remarkably responsive to phytoplankton blooms, indicating these bacteria could be particularly important in the processing of organic matter during such events. Our data suggest that quantitative and qualitative differences in phytoplankton species composition may lead to pronounced differences in bacterioplankton species composition.

* Corresponding author. Present address: Marine Microbiology, Department of Biology and Environmental Science, University of Kalmar, S-39182 Kalmar, Sweden. Phone: 46 (0)480 446000. Fax: 46 (0)480 447305. E-mail: jarone.pinhassi{at}hik.se.

{dagger} This is ELOISE contribution no. 496/40.

Applied and Environmental Microbiology, November 2004, p. 6753-6766, Vol. 70, No. 11
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.11.6753-6766.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Chen, L.-P., Xu, H.-Y., Fu, S.-Z., Fan, H.-X., Zhou, Y.-G., Liu, Z.-P. (2009). Lishizhenia tianjinensis sp. nov., isolated from coastal seawater. Int. J. Syst. Evol. Microbiol. 59: 2400-2403 [Abstract] [Full Text]  
  • Pinhassi, J., Nedashkovskaya, O. I., Hagstrom, A., Vancanneyt, M. (2009). Winogradskyella rapida sp. nov., isolated from protein-enriched seawater. Int. J. Syst. Evol. Microbiol. 59: 2180-2184 [Abstract] [Full Text]  
  • Guadayol, OÒs., Marrase, C., Peters, F., Berdalet, E., Roldan, C., Sabata, A. (2009). Responses of coastal osmotrophic planktonic communities to simulated events of turbulence and nutrient load throughout a year. J PLANKTON RES 31: 583-600 [Abstract] [Full Text]  
  • Hopkinson, B. M., Roe, K. L., Barbeau, K. A. (2008). Heme Uptake by Microscilla marina and Evidence for Heme Uptake Systems in the Genomes of Diverse Marine Bacteria. Appl. Environ. Microbiol. 74: 6263-6270 [Abstract] [Full Text]  
  • Bercovich, A., Vazquez, S. C., Yankilevich, P., Coria, S. H., Foti, M., Hernandez, E., Vidal, A., Ruberto, L., Melo, C., Marenssi, S., Criscuolo, M., Memoli, M., Arguelles, M., Mac Cormack, W. P. (2008). Bizionia argentinensis sp. nov., isolated from surface marine water in Antarctica. Int. J. Syst. Evol. Microbiol. 58: 2363-2367 [Abstract] [Full Text]  
  • Dimitriu, P. A., Pinkart, H. C., Peyton, B. M., Mormile, M. R. (2008). Spatial and Temporal Patterns in the Microbial Diversity of a Meromictic Soda Lake in Washington State. Appl. Environ. Microbiol. 74: 4877-4888 [Abstract] [Full Text]  
  • Gonzalez, J. M., Fernandez-Gomez, B., Fernandez-Guerra, A., Gomez-Consarnau, L., Sanchez, O., Coll-Llado, M., del Campo, J., Escudero, L., Rodriguez-Martinez, R., Alonso-Saez, L., Latasa, M., Paulsen, I., Nedashkovskaya, O., Lekunberri, I., Pinhassi, J., Pedros-Alio, C. (2008). From the Cover: Genome analysis of the proteorhodopsin-containing marine bacterium Polaribacter sp. MED152 (Flavobacteria). Proc. Natl. Acad. Sci. USA 105: 8724-8729 [Abstract] [Full Text]  
  • Mayali, X., Franks, P. J. S., Azam, F. (2008). Cultivation and Ecosystem Role of a Marine Roseobacter Clade-Affiliated Cluster Bacterium. Appl. Environ. Microbiol. 74: 2595-2603 [Abstract] [Full Text]  
  • Riemann, L., Leitet, C., Pommier, T., Simu, K., Holmfeldt, K., Larsson, U., Hagstrom, A. (2008). The Native Bacterioplankton Community in the Central Baltic Sea Is Influenced by Freshwater Bacterial Species. Appl. Environ. Microbiol. 74: 503-515 [Abstract] [Full Text]  
  • Van der Gucht, K., Cottenie, K., Muylaert, K., Vloemans, N., Cousin, S., Declerck, S., Jeppesen, E., Conde-Porcuna, J.-M., Schwenk, K., Zwart, G., Degans, H., Vyverman, W., De Meester, L. (2007). The power of species sorting: Local factors drive bacterial community composition over a wide range of spatial scales. Proc. Natl. Acad. Sci. USA 104: 20404-20409 [Abstract] [Full Text]  
  • Holmfeldt, K., Middelboe, M., Nybroe, O., Riemann, L. (2007). Large Variabilities in Host Strain Susceptibility and Phage Host Range Govern Interactions between Lytic Marine Phages and Their Flavobacterium Hosts. Appl. Environ. Microbiol. 73: 6730-6739 [Abstract] [Full Text]  
  • Lau, W. W. Y., Keil, R. G., Armbrust, E. V. (2007). Succession and Diel Transcriptional Response of the Glycolate-Utilizing Component of the Bacterial Community during a Spring Phytoplankton Bloom. Appl. Environ. Microbiol. 73: 2440-2450 [Abstract] [Full Text]  
  • Schafer, H. (2007). Isolation of Methylophaga spp. from Marine Dimethylsulfide-Degrading Enrichment Cultures and Identification of Polypeptides Induced during Growth on Dimethylsulfide. Appl. Environ. Microbiol. 73: 2580-2591 [Abstract] [Full Text]  
  • Lau, K. W. K., Ren, J., Wai, N. L. M., Qian, P.-Y., Wong, P.-K., Wu, M. (2006). Lishizhenia caseinilytica gen. nov., sp. nov., a marine bacterium of the phylum Bacteroidetes.. Int. J. Syst. Evol. Microbiol. 56: 2317-2322 [Abstract] [Full Text]  
  • Pinhassi, J., Bowman, J. P., Nedashkovskaya, O. I., Lekunberri, I., Gomez-Consarnau, L., Pedros-Alio, C. (2006). Leeuwenhoekiella blandensis sp. nov., a genome-sequenced marine member of the family Flavobacteriaceae.. Int. J. Syst. Evol. Microbiol. 56: 1489-1493 [Abstract] [Full Text]  
  • Alonso, C., Pernthaler, J. (2006). Concentration-dependent patterns of leucine incorporation by coastal picoplankton.. Appl. Environ. Microbiol. 72: 2141-2147 [Abstract] [Full Text]  
  • Pinhassi, J., Simo, R., Gonzalez, J. M., Vila, M., Alonso-Saez, L., Kiene, R. P., Moran, M. A., Pedros-Alio, C. (2005). Dimethylsulfoniopropionate Turnover Is Linked to the Composition and Dynamics of the Bacterioplankton Assemblage during a Microcosm Phytoplankton Bloom. Appl. Environ. Microbiol. 71: 7650-7660 [Abstract] [Full Text]  
  • Pernthaler, J., Amann, R. (2005). Fate of Heterotrophic Microbes in Pelagic Habitats: Focus on Populations. Microbiol. Mol. Biol. Rev. 69: 440-461 [Abstract] [Full Text]  
  • Pernthaler, A., Pernthaler, J. (2005). Diurnal Variation of Cell Proliferation in Three Bacterial Taxa from Coastal North Sea Waters. Appl. Environ. Microbiol. 71: 4638-4644 [Abstract] [Full Text]  
  • Reinthaler, T., Winter, C., Herndl, G. J. (2005). Relationship between Bacterioplankton Richness, Respiration, and Production in the Southern North Sea. Appl. Environ. Microbiol. 71: 2260-2266 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»