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Applied and Environmental Microbiology, November 2004, p. 6884-6886, Vol. 70, No. 11
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.11.6884-6886.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Evaluation of the LIVE/DEAD BacLight Kit for Detection of Extremophilic Archaea and Visualization of Microorganisms in Environmental Hypersaline Samples

Stefan Leuko,1 Andrea Legat,2 Sergiu Fendrihan,2 and Helga Stan-Lotter2*

Cyanobacteria and Astrobiology Research Laboratory, University of New South Wales, Sydney, New South Wales, Australia,1 Division of Molecular Biology, Department of Microbiology, University of Salzburg, Salzburg, Austria2

Received 19 February 2004/ Accepted 8 June 2004

ABSTRACT

Extremophilic archaea were stained with the LIVE/DEAD BacLight kit under conditions of high ionic strength and over a pH range of 2.0 to 9.3. The reliability of the kit was tested with haloarchaea following permeabilization of the cells. Microorganisms in hypersaline environmental samples were detectable with the kit, which suggests its potential application to future extraterrestrial halites.

* Corresponding author. Mailing address: Department of Molecular Biology, Hellbrunnerstr. 34, A-5020 Salzburg, Austria. Phone: 43 662 8044 5756. Fax: 43 662 8044 144. E-mail: helga.stan-lotter{at}sbg.ac.at.

Applied and Environmental Microbiology, November 2004, p. 6884-6886, Vol. 70, No. 11
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.11.6884-6886.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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