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Applied and Environmental Microbiology, November 2004, p. 6909-6913, Vol. 70, No. 11
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.11.6909-6913.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Detection and Enumeration of Vibrio vulnificus in Oysters from Two Estuaries along the Southwest Coast of India, Using Molecular Methods

Ammini Parvathi, H. Sanath Kumar, Indrani Karunasagar, and Iddya Karunasagar*

Department of Microbiology, College of Fisheries, University of Agricultural Sciences, Mangalore, India

Received 5 February 2004/ Accepted 2 June 2004

ABSTRACT

This study was conducted to understand the seasonal distribution of Vibrio vulnificus in oysters from two estuaries and the effect of environmental factors on the abundance of V. vulnificus in tropical waters. V. vulnificus was detected in 56.6% of the samples tested by colony hybridization with an alkaline phosphatase-labeled oligonucleotide probe (VV-AP), and the counts ranged from <10/g during the summer months to 103/g in the monsoon season at both sites. The density of V. vulnificus appeared to be controlled more by salinity than by temperature. A nested PCR used in this study detected V. vulnificus in 85% of the samples following 18 h of enrichment in alkaline peptone water.

* Corresponding author. Mailing address: University of Agricultural Sciences, Department of Fishery Microbiology, College of Fisheries, Mangalore 575 002, India. Phone: 91 824 2246384. Fax: 91 824 2246384. E-mail: mircen{at}sancharnet.in or karuna8sagar{at}yahoo.com.

Applied and Environmental Microbiology, November 2004, p. 6909-6913, Vol. 70, No. 11
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.11.6909-6913.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Warner, E., Oliver, J. D. (2008). Population Structures of Two Genotypes of Vibrio vulnificus in Oysters (Crassostrea virginica) and Seawater. Appl. Environ. Microbiol. 74: 80-85 [Abstract] [Full Text]  


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