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Applied and Environmental Microbiology, November 2004, p. 6914-6919, Vol. 70, No. 11
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.11.6914-6919.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Complete Type III Secretion System of a Mesophilic Aeromonas hydrophila Strain

Silvia Vilches,1 Cecilia Urgell,1 Susana Merino,1 Matilde R. Chacón,2 Lara Soler,2 Graciela Castro-Escarpulli,3 Maria Jose Figueras,2 and Juan M. Tomás1*

Departamento Microbiología, Facultad Biología, Universidad de Barcelona, Barcelona,1 Departament de Ciències Mèdiques Bàsiques, Facultat de Medicina i Ciències de la Salut, Universitat Rovira i Virgili, Reus, Spain,2 Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City, Mexico3

Received 30 April 2004/ Accepted 23 June 2004

ABSTRACT

We have investigated the existence and genetic organization of a functional type III secretion system (TTSS) in a mesophilic Aeromonas strain by initially using the Aeromonas hydrophila strain AH-3. We report for the first time the complete TTSS DNA sequence of an Aeromonas strain that comprises 35 genes organized in a similar disposition as that in Pseudomonas aeruginosa. Using several gene probes, we also determined the presence of a TTSS in clinical or environmental strains of different Aeromonas species: A. hydrophila, A. veronii, and A. caviae. By using one of the TTSS genes (ascV), we were able to obtain a defined insertion mutant in strain AH-3 (AH-3AscV), which showed reduced toxicity and virulence in comparison with the wild-type strain. Complementation of the mutant strain with a plasmid vector carrying ascV was fully able to restore the wild-type toxicity and virulence.

* Corresponding author. Mailing address: Departamento Microbiología, Facultad Biología, Universidad de Barcelona, Diagonal 645, 08071 Barcelona, Spain. Phone: 34-93-4021486. Fax: 34-93-4110592. E-mail: jtomas{at}ub.edu.

Applied and Environmental Microbiology, November 2004, p. 6914-6919, Vol. 70, No. 11
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.11.6914-6919.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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