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Applied and Environmental Microbiology, November 2004, p. 6936-6939, Vol. 70, No. 11
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.11.6936-6939.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Selection of Single-Chain Antibodies against the VP8* Subunit of Rotavirus VP4 Outer Capsid Protein and Their Expression in Lactobacillus casei

Vicente Monedero,1* Jesús Rodríguez-Díaz,2 Rosa Viana,1 Javier Buesa,2 and Gaspar Pérez-Martínez1

Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos (CSIC), Burjassot,1 Departamento de Microbiología, Facultad de Medicina, Universitat de València, Valencia, Spain2

Received 26 February 2004/ Accepted 12 July 2004

ABSTRACT

Single-chain antibodies (scFv) recognizing the VP8* fraction of rotavirus outer capsid and blocking rotavirus infection in vitro were isolated by phage display. Vectors for the extracellular expression in Lactobacillus casei of one of the scFv were constructed. L. casei was able to secrete active scFv to the growth medium, showing the potential of probiotic bacteria to be engineered to express molecules suitable for in vivo antirotavirus therapies.

* Corresponding author. Mailing address: Instituto de Agroquímica y Tecnología de Alimentos (CSIC), Apartado de Correos 73, 46100 Burjassot, Valencia, Spain. Phone: 34 963900022. Fax: 34 963636301. E-mail: btcmon{at}iata.csic.es.

Applied and Environmental Microbiology, November 2004, p. 6936-6939, Vol. 70, No. 11
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.11.6936-6939.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.





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